50 μL of patient serum 50 μL of diluent 50 µl of diluent 50 d. of diluent

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Fill in the (3) blanks with the correct dilution for each tube in the series.

50 μL of
patient
serum
50 μL of
diluent
50 μL of
diluent
50 pd. of
diluent
Transcribed Image Text:50 μL of patient serum 50 μL of diluent 50 μL of diluent 50 pd. of diluent
Expert Solution
Introduction:-

serial dilution is any dilution in which the concentration decreases by the same factor in each successive step.

In serial dilutions, you multiply the dilution factors for each step.

The dilution factor or the dilution is the initial volume divided by the final volume.

DF=ViVf

For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution,

DF=ViVf=1ml2ml. This is a 1:10 dilution.

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