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Predict the regions of low and high pH in the presence of oxygen in the mitochondria.
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- Biochemists working with isolated mitochondria recognize five energy "states" of mitochondria, depending on the presence or absence of essential substrates for respiration-O, ADP, oxidizable substrates, and so forth. The characteristics of each state are: state 1: mitochondria alone (in buffer containing P;) state 2: mitochondria + substrate, but respiration low due to lack of ADPThe pH values of the different compartments are shown below: matrix Intermembrane space Cytosol pH 7.8 – 8.0 pH ~ 7.0pH 7.0 – 7.4 Proton flow through ATP synthase leads to the formation of ATP, a process defined as the binding-change mechanism that was initially proposed by Boyer. Briefly explain Boyer’s binding change mechanism for the ATP synthase.Biochemists working with isolated mitochondria recognize five energy“states” of mitochondria, depending on the presence or absence of essential substrates for respiration—O2, ADP, oxidizable substrates, and so forth. The characteristics of each state are:state 1: mitochondria alone (in buffer containing Pi)state 2: mitochondria + substrate, but respiration low due to lack of ADPstate 3: mitochondria + substrate + limited amount of ADP, allowingrapid respirationstate 4: mitochondria + substrate, but all ADP converted to ATP, sorespiration slowsstate 5: mitochondria + substrate + ADP, but all O2 used up (anoxia),so respiration stops(a) On the graph, identify the state that might predominate in each stage of the trace indicated with a letter. (b) To determine whether isolated mitochondria exhibit respiratory control,one determines the ratio of rates of oxygen uptake in two different states.Which states?(c) Which state probably predominates in vivo in skeletal muscle fatiguedfrom a long…
- To examine: Whether the statement, "The three respiratory enzyme complexes in the mitochondrial inner membrane tend to associate with each other in ways that facilitate the correct transfer of electrons between appropriate complexes.", is true or false.Sonicating a suspension of mitochondria produces submitochondrial particles derived from the innermitochondrial membrane. These membranous vesicles seal inside out, so that the intermembrane space of themitochondria becomes the lumen of the submitochondrial particle. Explain (or diagram) the process of electrontransfer and oxidative phosphorylation in these particles. Assuming all the substrates for oxidativephosphorylation are present in excess, does ATP synthesis increase or decrease with an increase in the pH ofthe fluid in which the submitochondrial particles are suspended? Why?Can you please explain the relationship of ATP concentration and NADH concentration in this graph (cellular respiration)? At different treatments of ATP (E.g. At 25 ATP, 50 ATP, 100 ATP), why did the NADH concentration change?
- Using a generalized terminology (i.e. neither CIII nor CIV specific), explain how complex III and IV act as electron/proton charge separation device (not a proton pump) in mitochondria bioenergetics to create electron potential through the iner mitochondria membraneFor Krebs Cycle(Citric Acid Cycle) what are steps of cellular respiration for both aerobic (oxygen present) and anaerobic (without oxygen) respiration. what are the Inputs and Outputs: Clearly indicate the key substrates, intermediates, and products at each stage. Location: Show where this stage of cellular respiration occurs within the cell (e.g., cytoplasm, mitochondria). Energy Production: Highlight the ATP and NADH production at this stage. Oxygen: Show where oxygen is used in the process (e.g., ETC) and where carbon dioxide is produced. Electron Carriers: Indicate the role of electron carriers such as NAD+ and FAD in transferring eleDiagrams: Use diagrams or icons to represent the structures and molecules involved, such as the mitochondria, glucose, ATP, and oxygen. Key Information like important facts or formulas relevant to cellular respiration, like the chemical equations for each stage.b) Place a 'Yes' or a 'No' in the appropriate boxes that correspond to each listed feature of cellular respiration. You may need to state 'Yes' or 'No' in more than one box in a particular row in some cases. Cellular Respiration Feature Involved in aerobic respiration Occurs in the mitochondrial matrix Pyruvate molecules are produced Acetyl COA combines with a 4 carbon molecule Electrons are passed between protein carriers ATP is produced NAD+ gains hydrogen FADH₂ loses hydrogen Glycolysis Yes / No Krebs Cycle Yes / No Electron Transport chain Yes / No
- Discuss with reference to the biological role of glucose and oxygen, the process of aerobic cellular respiration.I need help woth this question. Aerobic respiration, used by the mitochondria of eukaryotic cells, is best represented by which of the following balanced equations? 6CO2 + 12H2S g C6H12O6 + 6S2 + 6H2O C6H12O6 + 6O2 + 6H2O g 6CO2 + 12H2O C12H22O11 + H2O g C6H12O6 + C6H12O6 6CO2 + 12H2O g C6H12O6 + 6O2 + 6H2O C6H12O6 + C6H12O6 g C12H22O11 + H2O