Predict the order of elution of the following compounds: 1-butanol, octylbenzene, 3-nitrooctane, 3-nitroheptane in Reversed-phase liquid chromatography
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- Predict the order of elution of the following compounds: 1-butanol, octylbenzene, 3-nitrooctane, 3-nitroheptane
- in Reversed-phase liquid chromatography
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- Which of the following pairs of compounds would be most easily separated by thin layer chromatography: n-octyl alcohol and 1-octene, hexadecane and octadecane, or chlorobenzene and bromobenzene?A thin-layer chromatography (TLC) experiement was run using three compunds: benzophenone, benzhydrol, and biphenyl. The three solvents that were used with the three compounds were hexane, ethyl acetate, and dichloromethane. Which solvent would be the best for separating these compounds and why?From the six analytes listed below, n-octanal, n-octane, 1-chlorooctane, n-octanol, n-heptane, and n-octanoic acid predict retention order (shortest to longest retention times) if the SP is: 100% polyethylene glycol or 100% dimethylpolysiloxane.
- A thin-layer chromatography (TLC) experiment was run using three compounds: benzophenone, benzhydrol, and biphenyl. The three solvents that were used with these compounds were hexane, ethyl acetate, and dichloromethane. Which solvent would be the best for separating these compounds and why?If a 0.0920 g sample of a 1:1 mixture of 4-nitroaniline and 2-nitroaniline was separated by column chromatography, and the recovered fractions weighed 0.0191 g ( 4-nitroaniline) and 0.0200 g (2-nitroaniline), what was the percent recovery of these two isomers? 20.6% 43.5% 21.7% 42.4%The following data were obtained by gas-liquid chromatography on a 40-cm packed column: Compound tr, min W, min Air 1.9 Methylcyclohexane 10.0 0.76 Methylcyclohexene 10.9 0.82 Toluene 13.4 1.06 If a resolution of 1.75 is desired in separating methylcyclohexane and methylcyclohexene (Use a spreadsheet to complete this question.) a. how many plates are required? 2798 plates b. how long must the column be if the same packing is used? Length = cm c. what is the retention time for methylcyclohexene on the column of part (b)? Retention time = min
- In an experiment of Reverse Phase chromatography using C-18 stationary phase and a mixture of methanol/ethyl acetate/acetic acid, a researcher tried to separate the 3 compounds shown below. Which compound would have the lowest Rf and which the highest Rf in this experiment? Explain your answer and show any relevant structures.Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.Draw a GC chromatogram for a 50:50 and 25:75 mixtures of hexane (bp 68°C) and toluene (bp 111°C).
- Look up the boiling point for each of the following compounds then determine the order that they will elute from the column. Use this elution order to identify the compound in each of the 6 standard chromatograms. Once you have identified the compound in each chromatogram, record its retention time. Compound Structure Compound Name Elution Order (1-6) Non polar → Polar Boiling Retention Time (From Chromatograms) Point Propyl acetate H H. H. Butyl acetate H. H. H H. H H. H. H. Methanol H O- Ethanol 2-butanone H H H. H. 4-methyl-2- pentanone H. H. HH H.Place the compounds in the order they will elute from the column when separated using reverse-phase liquid chromatography (RPLC). CH3 CH3 p-xylene napthalene CH3 anisole OH phenol anisole p-xylene Elutes first Elutes last Answer Bank naphthalene phenolA sample is going to be analyzed for trace amounts of ibuprofen using gas chromatography. Which of the following detectors would work best for the detection of ibuprofen? ibuprofen.png Group of answer choices alkali flame detector thermal conductivity detector flame ionization detector flame photometric detector sulfur chemiluminescence detector electron capture detector
