Predict the order of elution of the following compounds: 1-butanol, octylbenzene, 3-nitrooctane, 3-nitroheptane in Reversed-phase liquid chromatography
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- Predict the order of elution of the following compounds: 1-butanol, octylbenzene, 3-nitrooctane, 3-nitroheptane
- in Reversed-phase liquid chromatography
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- A thin-layer chromatography (TLC) experiement was run using three compunds: benzophenone, benzhydrol, and biphenyl. The three solvents that were used with the three compounds were hexane, ethyl acetate, and dichloromethane. Which solvent would be the best for separating these compounds and why?From the six analytes listed below, n-octanal, n-octane, 1-chlorooctane, n-octanol, n-heptane, and n-octanoic acid predict retention order (shortest to longest retention times) if the SP is: 100% polyethylene glycol or 100% dimethylpolysiloxane.A thin-layer chromatography (TLC) experiment was run using three compounds: benzophenone, benzhydrol, and biphenyl. The three solvents that were used with these compounds were hexane, ethyl acetate, and dichloromethane. Which solvent would be the best for separating these compounds and why?
- Look up the structures of naphthalene and benzoic acid and determine their polarities. Using the polarity of the molecules, predict which would elute first and which would elute second on a C18 column in reverse phase chromatography.If a 0.0920 g sample of a 1:1 mixture of 4-nitroaniline and 2-nitroaniline was separated by column chromatography, and the recovered fractions weighed 0.0191 g ( 4-nitroaniline) and 0.0200 g (2-nitroaniline), what was the percent recovery of these two isomers? 20.6% 43.5% 21.7% 42.4%The following data were obtained by gas-liquid chromatography on a 40-cm packed column: Compound tr, min W, min Air 1.9 Methylcyclohexane 10.0 0.76 Methylcyclohexene 10.9 0.82 Toluene 13.4 1.06 If a resolution of 1.75 is desired in separating methylcyclohexane and methylcyclohexene (Use a spreadsheet to complete this question.) a. how many plates are required? 2798 plates b. how long must the column be if the same packing is used? Length = cm c. what is the retention time for methylcyclohexene on the column of part (b)? Retention time = min
- Which one of the following chromatographic techniques is useful in the identification of organic molecules having molecular weights more than 20,000? 1) reverse phase chromatography 2) normal phase chromatography 3) high pressure liquid chromatography 4) gel permeation chromatographyUse a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.The following chromatogram was the output of the analysis of phytosterols in plants by gas chromatography. Beta-sitosterol Cholesterol Campesterol Stigmasterol Brassicasterol 16.5 17.0 17.5 18.0 18.5 Min Which compound had the highest affinity to the stationary phase? Last
- Draw a GC chromatogram for a 50:50 and 25:75 mixtures of hexane (bp 68°C) and toluene (bp 111°C).Look up the boiling point for each of the following compounds then determine the order that they will elute from the column. Use this elution order to identify the compound in each of the 6 standard chromatograms. Once you have identified the compound in each chromatogram, record its retention time. Compound Structure Compound Name Elution Order (1-6) Non polar → Polar Boiling Retention Time (From Chromatograms) Point Propyl acetate H H. H. Butyl acetate H. H. H H. H H. H. H. Methanol H O- Ethanol 2-butanone H H H. H. 4-methyl-2- pentanone H. H. HH H.Place the compounds in the order they will elute from the column when separated using reverse-phase liquid chromatography (RPLC). CH3 CH3 p-xylene napthalene CH3 anisole OH phenol anisole p-xylene Elutes first Elutes last Answer Bank naphthalene phenol
