PCR is a method used to amplify DNA. All you need is your DNA, primers (small pieces of DNA) complementary to two sites in your DNA (one on each strand), a heat-stable polymerase (enzyme that can make DNA according to a certain template), nucleotides and a thermo-cycler. By adding all the ingredients and heating and cooling the mixture in certain pre-programmed cycles, you can amplify the DNA between the two primers. At 100% efficiency, the number of double stranded DNA pieces is doubled in each cycle. Starting with 1 copy of double stranded DNA, how many copies will you have after 15 cycles at 100% efficiency? Answer:
PCR is a method used to amplify DNA. All you need is your DNA, primers (small pieces of DNA) complementary to two sites in your DNA (one on each strand), a heat-stable polymerase (enzyme that can make DNA according to a certain template), nucleotides and a thermo-cycler. By adding all the ingredients and heating and cooling the mixture in certain pre-programmed cycles, you can amplify the DNA between the two primers. At 100% efficiency, the number of double stranded DNA pieces is doubled in each cycle. Starting with 1 copy of double stranded DNA, how many copies will you have after 15 cycles at 100% efficiency? Answer:
Advanced Engineering Mathematics
10th Edition
ISBN:9780470458365
Author:Erwin Kreyszig
Publisher:Erwin Kreyszig
Chapter2: Second-order Linear Odes
Section: Chapter Questions
Problem 1RQ
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