order to help identify the things in a chromatogram, you can measure the Rf value for each spot. How would you work out the Rf value for each of the spots on the chromatogram above?
order to help identify the things in a chromatogram, you can measure the Rf value for each spot. How would you work out the Rf value for each of the spots on the chromatogram above?
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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In order to help identify the things in a chromatogram, you can measure the Rf value for each spot. How would you work out the Rf value for each of the spots on the chromatogram above?
Transcribed Image Text:The diagram illustrates the results of a simple thin layer chromatography (TLC) experiment. It shows a rectangular TLC plate placed inside an upright container, such as a beaker.
- **Solvent Front**: The diagram marks the "solvent front," which is the highest point reached by the solvent as it moves up the plate. This is indicated by a dashed line.
- **Spots**: Three distinct spots are visible on the TLC plate, aligned vertically. These represent different substances separated by the chromatography process:
- A red spot near the bottom, closest to the start line.
- Two distinct blue spots higher on the plate, showing further movement.
- **Start Line**: The origin of the sample application, indicated at the bottom of the plate with a small horizontal mark labeled "M."
The image effectively demonstrates how substances are separated based on their movement with the solvent across the stationary phase of the TLC plate.
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