Optimum cell concertation is very important for successful bone marrow culture. A technologist received 2.7 ml bone marrow specimen with concentration 20 x 10^6 cell/ml, she add 1ml this bone marrow to 9 ml Marrow-Max culture medium to set up a bone marrow culture. What is final cell concentration in this bone marrow culture?

Optimum cell concertation is very important for successful bone marrow culture. A technologist received 2.7 ml bone marrow specimen with concentration 20 x 10^6 cell/ml, she add 1ml this bone marrow to 9 ml Marrow-Max culture medium to set up a bone marrow culture. What is final cell concentration in this bone marrow culture?

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter12: Gravimetric Methods Of Analysis

Section: Chapter Questions

Problem 12.2QAP

See similar textbooks

Related questions

Q: Measure the mass of 0.400g of Co(NO3)2 + 6H2O and dissolve it in 15mL of distilled or deionized…

A: Limiting reagent is that reacts completely in the reaction. Number of moles of…

Q: Find the standard deviation (if the mass of KHP in three experimental solutions are 0.467,0.48, and…

Q: In a whole-cell patch-clamp experiment at 32 degrees C, the intracellular solution contains 16 mM…

A: In a membrane, the reversal potential of an ion is the membrane potential at which there is no…

Q: 3. The following mineral analysis was reported for a water sample taken from a local NJ well.…

Q: please highlight the correct answer

A: As per the question, a 10-6 is performed on a culture of bacteria in order to perform viable plate…

Q: You have to make 100mL of 0.05M HCI from a 10M HCI stock solution, but the only measuring devices…

A: Given that, Molarity of Stock HCl solution M1 = 10 M Molarity of dilute HCl solution,M2 = 0.05 M…

Q: Please answer as soon as possible. Thank you! A 10.000-g cocoa powder sample was dissolved in…

A: First of all, we have to convert the concentration of Fe given in micromolar to the grams of Fe in…

Q: Using C1V1=C2V2

A: The table given is,

Q: the differences between a Stober procedure and a water/oil emulsion procedure

A: In this question we have to describe the differences between a stober procedure and a water oil…

Q: 2+ as (6) A sample of an ore was analyzed for Cu* follows. A 1.25 g sample of the ore was dissolved…

A: Assuming the moles of Cu2+ in the ore is y. Given : Mass of ore = 1.25 g Volume of the solution 1…

Q: You are analyzing the effluent from a lotion plant for parabens using HPLC. You have a working…

A: The objective of this question is to determination of the concentration of parabens in the…

Q: 5. Which term is INCORRECTLY defined? a. linear range - the range over which the response of an…

A: All definitions of the terms that are widely used in analytical chemistry.

Q: A mixture of valine, glycine, isoleucine, aspartic acid, phenylalanine, arginine, lysine,…

Q: . What is the gravimetric factor of SO3 in BaSO4? 2. What is the normality of an oxidizing agent of…

A: The question is based on the concept of analytical chemistry. We have to calculate gravimetric…

Q: Find the ionic strength of a 8.0 mM of MgCl2. What are activities of Mg2 and Cl¹ ?

A: The objective of this question is to find the ionic strength of .

Q: A moving boundary experiment was performed at 25°C with 0.100 M KCI on the bottom and (initially)…

A: For K+:Measure of K+ particles (in moles) = (96.5 C/96485 C/equiv) *0.490For Na+Measure of Na+…

Q: How does capillary zone electrophoresis (CZE) works.

A: Capillary zone electrophoresis is a technique used for separating the individual components from a…

Q: A silver-30%gold alloy sample (cylindrical with 1 cm diameter and 0.3 cm height) is subjected to a…

A: The time can be calculated by Faraday's law, m = EitFm: mass of silveri: current (Ampere)F:…

Question

Optimum cell concertation is very important for successful bone marrow culture.

A technologist received 2.7 ml bone marrow specimen with concentration 20 x 10^6 cell/ml,

she add 1ml this bone marrow to 9 ml Marrow-Max culture medium to set up a bone

marrow culture. What is final cell concentration in this bone marrow culture?

Expert Solution

Step 1

Given that,

Initial concentration of bone marrow (C1)=20*10⁶cell/ml

Volume of this bone marrow was taken (V1) =1mL

In this 9mL Marrow-Max was added so final volume (V2)=1+9=10mL

Final concentration (C2)=?

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

3 ml of 15 x10^6 cells/ml bone marrow specimens was received. How many 10 ml bone marrow cultures with cell concentration 1.5 x 10^6 cells/ml you can set up?
A phlebotomist was acting as an instructor for a phlebotomy student from the local college and was anxious to help her learn all the special tests that had not been practiced in class. When a stat blood culture and electrolytes were ordered on a patient in the ICU, the instructor quickly grabbed the student and they headed for the floor. It was clear after looking at the patient that they would have to use a butterfly in a hand vein to collect the test specimen. The instructor was busy helping the student prepare the site and did not pay attention to the order the student layout all of the equipment/tubes/bottles for the draw. The student elated that she was able to access the difficult vein and that blood was flowing freely to the anaerobic bottles. After filling both it and the aerobic bottle to the proper level, she proceeded to draw a sst tube for the electrolytes. The blood flow was now very slow, with blood entering the tube one drop at a time. After patiently waiting for the…
In a similar experiment, the mass of 3 PB tablets was measured to be 3.1823 g. The tablets were crushed, and 2.9801 g of the powder was transferred to a beaker and reacted with HCl. After filtration, the filtrate was transferred to a 50-mL volumetric flask and diluted with water. 10.00 mL of this stock solution was combined with 0.2 M Na3PO4. The resulting precipitate weighed 0.2425 g after drying. What is the mass of BSS per tablet in mg?Round your answer to 1 decimal place.
Concentration of AR Stock Solution (ppmppm) 21.22 Unrounded Rounded Concentration of AR Stock Solution (μMμM) 42.7461 42.7
In the preparation of the standard curve for protein analysis, 50 mn BSA (bovine serum albumin) dissolved in H2O to a final volume of 5 mL was used as stock solution. What is the weight of BSA in 0.1 mL of stock solution? In 0.2 mL?
The standard addition method is used to analyze a sample of a river water for mercury. Solution A is made by pipetting 5.00 mL of undiluted sample in to a 10 mL volumetric flask and filling to the mark with DI water. Solution B is made by pipetting 5.00 mL of undiluted sample and 3.00 mL of 15.0 ppb of Hg standard into same 10.0 mL volumetric flask and filling to the mark with DI. Solution A and B are analyzed using atomic absorption spectroscopy and give a percent transmittance values of 56 % and 33 % respectively (not blank corrected). A blank has a transmittance of 96%. What is the corrected absorbance of both solution A and B? A. Solution A: 0.123 Solution B: 0.463 B. Solution A: 0.463 Solution B: 0.234 C. Solution A: 0.123 Solution B: 0.234 D. Solution A: 0.234 Solution B: 0.463
1. Straight lime softening A groundwater was analyzed and found to have the following composition (all concentrations are as CaCO): Estimate the lime dose (in mg 1.) required to soften the water. HCO 155mg/1 Ca 210 mg Mg-15mg/1 Alk.-260 ml Temp-10C 2. Excess lime softening A groundwater was analyzed and found to have the following composition (all concentrations are as CaCO)). Estimate the lime dose (in mg/L.) required to soften the water.. H:CO 155mg/1 Ca 180 mg/1 Mg-60mg/1 Alk. -260 mg/1 Temp-10C
Assume the data below was collected for two different sample solutions using the same experimental procedure you carried out. Based on the two lines shown below, which solution (orange data points or blue data points) corresponds to the sample with the higher molar absorptivity? 0.9 y4.771 - 0.0728 0.7 0.6 04 0.3 02 y1414 - 0.0546 R0.99642 0.1 0.02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 0.18 02 0.1 [Concentration, M] orange data points (lower line) O blue data points (upper line) Absorbance
You are analyzing the effluent from a lotion plant for parabens using HPLC. You have a working standard solution with a concentration of 59.4 ppm which returned a Peak Height of 10,164 and Peak Area of 5,315. You take 44.4 L of the water sample and concentrate it to 0.3 L and analyze this solution. The Peak Height is 9,119 and the Peak Area is 6,320. What is the concentration of parabens in the effluent of the plant in mg/L to two decimal places.
Table 2: Absorbance Values of Standards and Unknowns. Sample Blank Standard Solution 1 Standard Solution 2 Standard Solution 3 Standard Solution 4 Standard Solution 5 Water Sample 1 Water Sample 2 Phosphate Concentration (in ppm) Y17 0 ppm 0.02 ppm 0.04 ppm 0.08 ppm 0.16 ppm 0.32 ppm Freeman Lake 0.001 0.325 0.292 0.413 0.315 0.039 0.054 0.049 Absorbance at 2= 690 nm Calculations: 1. Construct a phosphate standard curve in Excel by plotting concentration (in ppm) on your x-axis and Absorbance (unitless) on your y-axis for your known solutions. Label the axes on the graph and provide the curve with a title. Use a linear trendline to generate a best fit line to your data. Label the graph with the equation and the R" value. Insert your labeled graph in the space below. X
If the cells were spread out into a thin layer that was a single cell thick, what would be the total surface area ( in square meters) for the top side of the cell layer ? Be sure your answer has the correct number of significant digits? ……m^2pls help me
The Ksp of BaSO4 is 1.7 x 10-10. Calculate the concentration of the Ba+2 and SO4-2.