In a whole-cell patch-clamp experiment at 32 degrees C, the intracellular solution contains 16 mM NaCl, 150 mM K-gluconate, and 1µM Ca(NO3)2, and the extracellular solution contains 150 mM NaCl, 3 mM KCI, 25 mM NaHCO3, 2 mM MgCl2, and 1.5 mM CaCl2. The below graph is a current-voltage (I-V) curve generated by measuring the current fluy thrOUgh a spocific jon chappel over

In a whole-cell patch-clamp experiment at 32 degrees C, the intracellular solution contains 16 mM NaCl, 150 mM K-gluconate, and 1µM Ca(NO3)2, and the extracellular solution contains 150 mM NaCl, 3 mM KCI, 25 mM NaHCO3, 2 mM MgCl2, and 1.5 mM CaCl2. The below graph is a current-voltage (I-V) curve generated by measuring the current fluy thrOUgh a spocific jon chappel over

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Concept explainers

Potentiometric Titrations

Potentiometric titration is one of the important titration methods and is mainly carried out to characterize acid solutions. It follows a method similar to that of the normal titration technique, but the main difference is that there is no indicator used in the potentiometric titrations. Instead of using an indicator, electrodes are employed for monitoring the whole titration process. A scientist named Robert Behrend did the potentiometric titration for the first time, and it was done using the mercurous solution and potassium chloride.

Question

In a whole-cell patch-clamp experiment at 32
degrees C, the intracellular solution contains
16 mM NaCl, 150 mM K-gluconate, and 1µM
Ca(NO3)2, and the extracellular solution
contains 150 mM NaCl, 3 mM KCI,, 25 mM
NaHCO3, 2 mM MgCl2, and 1.5 mM CaCl2.
The below graph is a current-voltage (I-V)
curve generated by measuring the current
flux through a specific ion channel over a
range of voltages (-100 to +40mV).
What is the reversal potential of this current?
1000 -
800
600
400-
200
-100 -80
-60
-40
-20
20
40
-200
mv
-400
pA

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