One of your colleagues in a molecular laboratory did not put proper labels to some of the vessels he used in his experiments. Because of that, you accidentally mixed the engineered plasmid DNA to a solution contained in a tube labelled with just "A". Apparently, that tube ("A") contains chromosomal genetic material, some RNA fragments, some glucose sugar molecules, some peptides, and long chain proteins from cell debris. You remember that the engineered plasmid will give you the chance to express a very important protein that can help cure Covid-19 patients. How do you get the plasmid from the mixed solution and bring it to its pure plasmid form (devoid of any unwanted materials for your downstream processes). Outline a step by step procedure on this and please make sure to include a step where you can prove that the content of the final vessel is indeed the engineered plasmid.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
One of your colleagues in a molecular laboratory did not put proper labels to some of the vessels he used in his experiments. Because of that, you accidentally mixed the engineered plasmid DNA to a solution contained in a tube labelled with just "A". Apparently, that tube ("A") contains chromosomal genetic material, some RNA fragments, some glucose sugar molecules, some peptides, and long chain proteins from cell debris. You remember that the engineered plasmid will give you the chance to express a very important protein that can help cure Covid-19 patients. How do you get the plasmid from the mixed solution and bring it to its pure plasmid form (devoid of any unwanted materials for your downstream processes). Outline a step by step procedure on this and please make sure to include a step where you can prove that the content of the final vessel is indeed the engineered plasmid.
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