NO EXCEL! Please help me to understand to how calculate this exercise without using excel. I'm not allowed to use computer during exam, and I only have learned to calculate these type of exercises by using excel. If you can write as much detail as possible I will be thankful. Exercise 3: Calculations of Enzyme KineticsProblem 1The table below shows average values of glucose product concentrations [P] in mM obtained atvarious times for a pure saccharase (EC 3.2.1.26) acting on sucrose in reactions run at five differentinitial substrate concentrations [So] in mM. All other reaction conditions, except for the [So] weresimilar for the reactions.Time (minutes)0124[So] 10 mM [So] = 15 mM [S] = 25 mM=01.032.104.046.0601.082.154.226.2501.122.194.306.44[So] = 40 mM01.152.234.366.55a. Estimate the initial rate, vi, at each initial substrate concentration assuming that only the dataobtained during the first 4 minutes are linear.b. Estimate the KM and Vmax for the saccharase hydrolysis of sucrose.c. Comment on the assay design with respect to the [So] and the extents of reaction obtained inthe different reactions in relation to the results obtained.

The initial rate of a reaction ( Vi ) is the amount of product generated within a period of time.…

Answered: Estimate the initial rate, vi, at each…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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NO EXCEL! Please help me to understand to how calculate this exercise without using excel. I'm not allowed to use computer during exam, and I only have learned to calculate these type of exercises by using excel.

If you can write as much detail as possible I will be thankful.

Exercise 3: Calculations of Enzyme Kinetics
Problem 1
The table below shows average values of glucose product concentrations [P] in mM obtained at
various times for a pure saccharase (EC 3.2.1.26) acting on sucrose in reactions run at five different
initial substrate concentrations [So] in mM. All other reaction conditions, except for the [So] were
similar for the reactions.
Time (minutes)
0
1
2
4
[So] 10 mM [So] = 15 mM [S] = 25 mM
=
0
1.03
2.10
4.04
6.06
0
1.08
2.15
4.22
6.25
0
1.12
2.19
4.30
6.44
[So] = 40 mM
0
1.15
2.23
4.36
6.55
a. Estimate the initial rate, vi, at each initial substrate concentration assuming that only the data
obtained during the first 4 minutes are linear.
b. Estimate the KM and Vmax for the saccharase hydrolysis of sucrose.
c. Comment on the assay design with respect to the [So] and the extents of reaction obtained in
the different reactions in relation to the results obtained.

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Is it possible to determine Km and Vmax without using excel? Because we are not allowed to use computer during exam, so I dont understand how its done without using excel?

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