Normal calibration curves are generally run in water (pure solvent). If there is something in your unknown sample "matrix" which affects the analytical signal, you'll report an incorrect concentration. In this situation you'll choose to run a "standard addition" calibration curve. Standard addition entails adding the same amount of unknown to each of your standard solutions prior to analysis. This transforms your independent variable from "concentration" to "added concentration". These leads to an entirely different calculation for your unknown concentration. A standard addition procedure can be used with any analytical method. In this problem we're conducting a fluorescence assay for a blood protein which is a cancer marker. For the following problem plot a standard addition curve from the data shown below, and calculate the concentration for your unknown. Your unknown number is: 770 Solution 1 is your unknown diluted 25:1 and has an intensity of 5.94 Solution 2 is your unknown diluted 25:1 plus 25 ng/mL of standard and has an intensity of 9.09 Solution 3 is your unknown diluted 25:1 plus 50 ng/mL of standard and has an intensity of 12.22 Solution 4 is your unknown diluted 25:1 plus 75 ng/mL of standard and has an intensity of 15.15 Solution 5 is your unknown diluted 25:1 plus 100 ng/mL of standard and has an intensity of 18.44 Use a spreadsheet to produce a standard addition calibration curve graph with a linear least square calculation of the best fit line. Calculate the following in CLEARLY LABELLED cells in your spreadsheet. The slope = The intercept = The R2 value = The fluorescent marker concentration of the unknown you were given at the start of the experiment = ng/mL
Normal calibration curves are generally run in water (pure solvent). If there is something in your unknown sample "matrix" which affects the analytical signal, you'll report an incorrect concentration. In this situation you'll choose to run a "standard addition" calibration curve. Standard addition entails adding the same amount of unknown to each of your standard solutions prior to analysis. This transforms your independent variable from "concentration" to "added concentration". These leads to an entirely different calculation for your unknown concentration. A standard addition procedure can be used with any analytical method. In this problem we're conducting a fluorescence assay for a blood protein which is a cancer marker. For the following problem plot a standard addition curve from the data shown below, and calculate the concentration for your unknown. Your unknown number is: 770 Solution 1 is your unknown diluted 25:1 and has an intensity of 5.94 Solution 2 is your unknown diluted 25:1 plus 25 ng/mL of standard and has an intensity of 9.09 Solution 3 is your unknown diluted 25:1 plus 50 ng/mL of standard and has an intensity of 12.22 Solution 4 is your unknown diluted 25:1 plus 75 ng/mL of standard and has an intensity of 15.15 Solution 5 is your unknown diluted 25:1 plus 100 ng/mL of standard and has an intensity of 18.44 Use a spreadsheet to produce a standard addition calibration curve graph with a linear least square calculation of the best fit line. Calculate the following in CLEARLY LABELLED cells in your spreadsheet. The slope = The intercept = The R2 value = The fluorescent marker concentration of the unknown you were given at the start of the experiment = ng/mL
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:Normal calibration curves are generally run in water (pure solvent). If there is something in your unknown sample "matrix" which affects the analytical signal, you'll report an
incorrect concentration. In this situation you'll choose to run a "standard addition" calibration curve. Standard addition entails adding the same amount of unknown to each of
your standard solutions prior to analysis. This transforms your independent variable from "concentration" to "added concentration". These leads to an entirely different
calculation for your unknown concentration. A standard addition procedure can be used with any analytical method. In this problem we're conducting a fluorescence assay for
a blood protein which is a cancer marker.
For the following problem plot a standard addition curve from the data shown below, and calculate the concentration for your unknown.
Your unknown number is: 770
Solution 1 is your unknown diluted 25:1 and has an intensity of 5.94
Solution 2 is your unknown diluted 25:1 plus 25 ng/mL of standard and has an intensity of 9.09
Solution 3 is your unknown diluted 25:1 plus 50 ng/mL of standard and has an intensity of 12.22
Solution 4 is your unknown diluted 25:1 plus 75 ng/mL of standard and has an intensity of 15.15
Solution 5 is your unknown diluted 25:1 plus 100 ng/mL of standard and has an intensity of 18.44
Use a spreadsheet to produce a standard addition calibration curve graph with a linear least square calculation of the best fit line. Calculate the following in CLEARLY LABELLED
cells in your spreadsheet.
The slope
%D
The intercept =
The R2 value =
The fluorescent marker concentration of the unknown you were given at the start of the experiment
ng/mL
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