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In a denaturing protein electrophoresis, what might be the
Why a particular protein did not migrate to the anode? How
it can be fixed?
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- Define SDS gel electrophoresis.In zone electrophoresis, why is it possible to isolate the components of a sample and recover them for identification? a) what influences the direction of migration of the components of a sample in zone electrophoresis?Name the substance used as a medium in gel electrophoresis.
- What side of the electrophoresis chamber (red or black) should the wells be on when the gel is placed in the chamber? What would happen if you placed the agarose gel with the wells on the opposite side of the chamber?In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?Why is polyacrylamide gel is used in electrophoresis?
- What is the principle of the gel electrophoresis separation technique?What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.For the pour plate method, state which plate will be chosen for colony isolation and why? From the chosen plate, indicate (circle) the colonies for both bacterial species that will be selected and transferred to new nutrient agars as a pure culture.