Explain what a single colony represents on the petri dish labeled + LB/AMP. Plate LB-Plasmid LB + Plasmid LB/amp - Plasmid Yes Yes No + LB LB/amp + Plasmid Yes LB Prediction Reeson Plate only contains Yes food/bacteria Plate only contains Yes food/bacteria Bacteria will be killed by ampicillin Bacteria has ampicillin resistance LB Click on pic to zoom Click on pic to zoom -LB Observed Result No Yes chlamp x Click on pic to zoom + LB/AMP Blamp Click on pic to zoom - LB/AMP
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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LB-Plasmid
LB + Plasmid
LB/amp - Plasmid
Yes
Yes
No
+ LB
LB/amp + Plasmid Yes
LB
Prediction
Reeson
Plate only contains Yes
food/bacteria
Plate only contains Yes
food/bacteria
Bacteria will be
killed by ampicillin
Bacteria has
ampicillin
resistance
LB
Click on pic to zoom Click on pic to zoom
-LB
Observed Result
No
Yes
chlamp
x
Click on pic to zoom
+ LB/AMP
Blamp
Click on pic to zoom
- LB/AMP"
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