immunization and diagnostic fill out the puzzle Immunization and Diagnostics Puzzle11/10/21, 1 13 PM1110141516181719DownAcross2. Measles, mumps and rubella1. Immunoglobulin derived from human donors4. Long-term protection capable of re-stimulation5. Used in selective media to identify halophiles3. A diagnostic screen for HIV antibodies5. Urine, feces, saliva6. An early method of vaccination using infectedmaterial from a patient8. A delivery route of active immunization11. The TORCH test is an example7. His discovery led to methods of vaccination 12. An estimate of the amount of antibodies orviruses in a solution9. The unwanted introduction of microbes into anenvironment or procedure10. Most safe and effective means of preventinginfectious disease13. Neither anti-A or anti-B antibodies14. The act of growing microbes15. Administered with adjuvants16. An antibody used for human therapy18. A sample introduced into a collection ofnutrients17. A vaccine with no reversion or mutation19. A population of cells from a single cell

ACROSS- 2- 4- LATENT 5- SALT 8- INTRAMUSCULAR 11- 12- TITRE 13- ANTI-D ANTIBODIES. 14- CULTURE 16-…

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Anatomy and Physiology

mmunization and diagnostic fill out the puzzle

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Describe the background aims of immunologic testing.
Method: 1. 96 well plates are coated with an antigen. Sites unoccupied by the antigen are blocked with a blocking buffer to prevent non-specific binding of sample. Draw this below: 2. Serum samples are added and incubated to allow specific antibodies to bind to specific antigen. Antibodies that do not recognize and bind specifically to the antigen remain unbound. Draw this below: Positive sample Negative sample 3. Non-specific antibodies that do not bind to the antigen are washed away. Draw this below. Positive sample Negative sample 4. An enzyme labeled (e.g. alkaline phosphatase or horseradish peroxidase) secondary antibody is incubated with the samples and binds tightly to sample antibody. Draw this below.
Compare and contrast the immunodeficiency observed in SCIDpatients to that of AIDS patients. What cell types are affectedby each condition?
1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge true/false 3) PBST is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8
Differentiate among lytic infection, latent infection, chronic infection,and transformation.