Met-Val-Ser-Thr-Lys Val-le-Trp-Thr–Leu-Met-lle Leu-Phe-Asn-Glu-Ser-Arg The sequences of the smaller peptides produced by chymotryp- sin digestion were as follows: Asn-Glu-Ser-Arg-Val-lle-Trp Thr-Leu-Met-Ile Met-Val-Ser–Thr–Lys-Leu–Phe Deduce the sequence of the original peptide.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter1: The Clinical Laboratory
Section1.7: The Metric System
Problem 4RQ
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A sample of a peptide of unknown sequence
was treated with trypsin; another sample of the same peptide was
treated with chymotrypsin. The sequences (N-terminal to C-terminal)
of the smaller peptides produced by trypsin digestion were as follows:

Met-Val-Ser-Thr-Lys
Val-le-Trp-Thr–Leu-Met-lle
Leu-Phe-Asn-Glu-Ser-Arg
The sequences of the smaller peptides produced by chymotryp-
sin digestion were as follows:
Asn-Glu-Ser-Arg-Val-lle-Trp
Thr-Leu-Met-Ile
Met-Val-Ser–Thr–Lys-Leu–Phe
Deduce the sequence of the original peptide.
Transcribed Image Text:Met-Val-Ser-Thr-Lys Val-le-Trp-Thr–Leu-Met-lle Leu-Phe-Asn-Glu-Ser-Arg The sequences of the smaller peptides produced by chymotryp- sin digestion were as follows: Asn-Glu-Ser-Arg-Val-lle-Trp Thr-Leu-Met-Ile Met-Val-Ser–Thr–Lys-Leu–Phe Deduce the sequence of the original peptide.
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