Make a standard calibration curve wherein the x-axis is the total concentration of standard 1 to 6 and the y axis is their corresponding corrected absorbance ..(Note never include the absorbance reading of the unknown sample when making a standard curve) Absorbance Reading Test Tube / Standard Glucose Concentration (mg/mL) Initial Absorbance Corrected Absorbance Blank 0.00 0.045 0.000 Standard 1 1.00 0.102 0.057 Standard 2 2.00 0.236 0.191 Standard 3 4.00 0.697 0.652 Standard 4 6.00 1.104 1.059 Standard 5 8.00 1.385 1.340 Standard 6 10.0 1.915 1.870 Unknown 2.42 0.361 0.316 Data Set for Quantitative Analysis Tube No. ABSORBANCE TRIAL 1 TRIAL 2 TRIAL 3 TRIAL 4 TRIAL 5 TRIAL 6 1 0.041 0.044 0.046 0.046 0.046 0.046 2 0.102 0.103 0.103 0.102 0.102 0.102 3 0.236 0.235 0.240 0.235 0.237 0.235 4 0.703 0.698 0.696 0.694 0.693 0.695 5 1.102 1.100 1.11 1.101 1.103 1.07 6 1.39 1.386 1.384 1.383 1.38 1.385 7 1.915 1.920 1.917 1.913 1.912 1.911 8 0.355 0.356 0.357 0.360 0.358 0.377
Make a standard calibration curve wherein the x-axis is the total concentration of standard 1 to 6 and the y axis is their corresponding corrected absorbance ..(Note never include the absorbance reading of the unknown sample when making a standard curve)
Absorbance Reading
|
Test Tube / Standard |
Glucose Concentration (mg/mL) |
Initial Absorbance |
Corrected Absorbance |
|
Blank |
0.00 |
0.045 |
0.000 |
|
Standard 1 |
1.00 |
0.102 |
0.057 |
|
Standard 2 |
2.00 |
0.236 |
0.191 |
|
Standard 3 |
4.00 |
0.697 |
0.652 |
|
Standard 4 |
6.00 |
1.104 |
1.059 |
|
Standard 5 |
8.00 |
1.385 |
1.340 |
|
Standard 6 |
10.0 |
1.915 |
1.870 |
|
Unknown |
2.42 |
0.361 |
0.316 |
Data Set for Quantitative Analysis
|
Tube No. |
ABSORBANCE |
|||||
|
TRIAL 1 |
TRIAL 2 |
TRIAL 3 |
TRIAL 4 |
TRIAL 5 |
TRIAL 6 |
|
|
1 |
0.041 |
0.044 |
0.046 |
0.046 |
0.046 |
0.046 |
|
2 |
0.102 |
0.103 |
0.103 |
0.102 |
0.102 |
0.102 |
|
3 |
0.236 |
0.235 |
0.240 |
0.235 |
0.237 |
0.235 |
|
4 |
0.703 |
0.698 |
0.696 |
0.694 |
0.693 |
0.695 |
|
5 |
1.102 |
1.100 |
1.11 |
1.101 |
1.103 |
1.07 |
|
6 |
1.39 |
1.386 |
1.384 |
1.383 |
1.38 |
1.385 |
|
7 |
1.915 |
1.920 |
1.917 |
1.913 |
1.912 |
1.911 |
|
8 |
0.355 |
0.356 |
0.357 |
0.360 |
0.358 |
0.377 |
![Example only!
Glucose Standard Curve
0,9
0,8
y=mx + b
R? =_
0,7
0,6
0,5
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0,1
0,5
1,5
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2,5
-0,1 0
[Glucose] (ug/mL)
Absorbance at 480 nm](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F6eba7c3b-5035-4c5e-a76a-1c728139f79f%2Fe1267482-0fbf-4d28-9f98-def560b7f1b6%2Fqx0x3oi_processed.png&w=3840&q=75)
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