lgal oil from Spirogyra sp. was extracted using a combined technology of partitioning between phase extraction as shown in Figure Q.1. (a) Suitable extraction technique to extract the oil from the algal suspension as shown in the first step. (b) Suitable extraction techniques to separate the aqueous layer, algal protein suspension particle and the organic layer on the third step. Please explain in detail each of the steps. (c) Suitable evaporation technique for separate algal oil from the organic layer in the fourth step.

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Algal oil from Spirogyra sp. was extracted using a combined technology of partitioning between phase extraction as shown in Figure Q.1. (a) Suitable extraction technique to extract the oil from the algal suspension as shown in the first step. (b) Suitable extraction techniques to separate the aqueous layer, algal protein suspension particle and the organic layer on the third step. Please explain in detail each of the steps. (c) Suitable evaporation technique for separate algal oil from the organic layer in the fourth step.
First Step
Three phases' form
Second Step
+(NH,),SO4
+tert-butanol
Oil in tert-
butanol
Algal protein
Aqueous
layer
Aqueous
algal
suspension
Third Step
Organic
layer
separated
Fourth Step
Algal oil
Figure Q.1: Extraction of algal oil by Three-Phase Partitioning (TPP).
Transcribed Image Text:First Step Three phases' form Second Step +(NH,),SO4 +tert-butanol Oil in tert- butanol Algal protein Aqueous layer Aqueous algal suspension Third Step Organic layer separated Fourth Step Algal oil Figure Q.1: Extraction of algal oil by Three-Phase Partitioning (TPP).
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