Can you write areport on ProteinElectrophoresis,Q&A pleaseExperiment No. (7)Title: Protein ElectrophoresisAim of Experiment: Determination of individualprotein in serumTheory:One of the simple techniques for quantitation of serum protein istheir separation into an electric field and this procedure is referred to as"serum protein electrophoresis" (SPE). Electrophoresis is widely used forseparating protein fractions. In addition to separating albumin fromglobulins this technique further fractionates the globulins into four or fivemajor group: alphal (a1), alpha2 (a2) or betal, beta2 (B1 , B2) and gamma(Y) globulins.Electrophoresis is similar in many ways to chromatography, exceptthat separation is due to the movement of charged particles through anelectric current. If these particles are charged differently they will movein opposite directions, the positively charged particles to the cathode andthe negatively charged to the anode. In this separation technique abuffered medium at a fixed pH is essential to ensure that the charge onthe particles and therefore the rate of migration is stabilized.Electrophoresis is especially usedmacromolecular substances such as proteins and lipids, although it isapplicable to many other substances as well. In routine clinical testing thevarious protein substances in human serum are separated and quantitated,enzymes are separated and quantitated, enzymes are separated andidentified, and various hemoglobin fractions are identified. In additionisolation and quantitation of lipoproteins can also be carried out. Theabove substances contain numerous fractions of similar mobility,therefore, when submitted to electrophoresis, the sharpness of separationwill depend upon the extent to which each fraction is homogeneous in itsmigration. Each fraction carries a net charge which varies with the pH ofthe environment.for the separation ofThe direction and speed of electrophoresis depends upon severalfactors such as (1) net charge of the molecule, (2) size and shape of themolecule, (3) electric field strength, (4) nature of the supporting medium,and (5) the temperature of operation. One of the important factors aboveis the pH of the buffer solution. The most commonly used pH for proteinelectrophoresis is pH 8.6, under these conditions most proteins arenegative and therefore migrate toward the anode.

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Can you write a report on Protein Electrophoresis, |Q&A please Aim of Experiment: Determination of individual Experiment No. (7) Title: Protein Electrophoresis protein in serum Theory: One of the simple techniques for quantitation of serum protein is their separation into an electric field and this procedure is referred to as

Can you write a report on Protein Electrophoresis, |Q&A please Aim of Experiment: Determination of individual Experiment No. (7) Title: Protein Electrophoresis protein in serum Theory: One of the simple techniques for quantitation of serum protein is their separation into an electric field and this procedure is referred to as

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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