Answered: lease describe a strategy to correctly…

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter9: Parenteral Medication Labels And Dosage Calculation

Section: Chapter Questions

Problem 4.4P

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A scientist tries to harvest bacterial cells by centrifugation; he used an RPM of 5000. If the radius of the rotor is 144 mm, the relative centrifugal force is 40248 x g 280x 4025 K 402.5 x Question 16 The recipe for making 1 L of LB broth is 10 g Tryptone, 5 g yeast extract, and 10 g sodium chloride. The amount of each substance if you are making 100 mL of LB broth. A Tryptone, 005 yeast extract, and is sodium chloride 0.1 g Tryptone, 0.5 g yeast extract, and 0.1 g sodium chloride 701 Tryptone, 0.05 g yeast extract, and 0.1 g sodium chloride 1 Tryptone 0.5 yeast extract and 1 sodium chlade
You want to add cells at a density of 3 x 104 cells / cm2 to a 12-well plate where each well has an area of 4 cm 2 . Your cell suspension contains 6 x 104 cells / ml. What volume of this suspension would you add to the well to achieve the desired cell density?
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3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTES
You have been charged with design of a liner to be used in a cell for a solid waste landfill in Virginia. The cell you will be designing is approximately square and covers 10 hectares. New regulations in the state permit a performance-based design under provisions of RCRA Subtitle D. According to these regulations, two liner systems, an earthen liner and a composite liner, were proposed. The earthen liner includes a 60 cm clay liner compacted in 4 lifts, whereas the proposed composite liner consists of a 60 mil HDPE geomembrane placed on the CCL. In the first phase of the study, the engineer managing the project requested that the toxicologists and hydrogeologists working for her determine an acceptable mass loading to the aquifer, which underlies the new cell. Two contaminants of concern were chloride (Cl- ) and dichloromethane (methylene chloride). The analysis showed that the mass loading for chloride cannot exceed 250 tons/year within 100 years. For methylene chloride, the maximum…
If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperation
Develop a spreadsheet to predict as functions of time with concentration, volimme and permeate flows for a batch ultrafiltration. Assume that the flux depends on concentration as follows: J(L/M²/H) = A*EXP(-bC) where C is the retentate concentration in g/l. Assume that the recirculation rate is kept high enough that shear rate dependence is eliminated. Do a sample run with C.= 0.5 g/l, Cfinal = 50 g/l, A = 50, and b = 0.02. Assume a filtration area of 19.4M² and an initial volume of 3255 L. Assume σ (protein) = 1.0 or σ (protein) = 0.95 or σ (protein) = 0.90.
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Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.206
18
Put the following steps for running a gel in order. first Remove comb and put gel int v [ Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel second Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn v

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lease describe a strategy to correctly balance a centrifure rotor if you have 4 samples. 2 samples with 100 mL volume and 2 samples with 50 mL volume