Kinesin-1 was the first member of the kinesin superfamily to be identified and therefore is perhaps the best-characterized superfamily member. What fundamental property of kinesin was used to purify it?
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Kinesin-1 was the first member of the kinesin superfamily to be identified and therefore is perhaps the best-characterized superfamily member. What fundamental property of kinesin was used to purify it?
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- What was the first member of the kinesin su-perfamily to be identified and therefore is perhaps the best-characterized superfamily member. What fundamentalproperty of kinesin was used to purify it?Yeast cells are eukaryotes, and they require a functional secretory pathway to grow and to maintain cellular organization. But surprisingly, when SRP is removed from yeast cells by deleting the relevant genes, the cells are still alive (although they grow slowly). a) How can yeast survive without SRP? Propose two alternative hypotheses. b) How might electron microscopy of normal and SRP-deficient yeast cells help you to distinguish between these two hypotheses?What feature of procollagen synthesis provided early evidence for the Golgi cisternal maturation model?
- Genetic testing of a 29-month-old boy shows a mutation in the Phe residue at position 508 in the cystic fibrosis transmembrane conductance regulator (CFTCR) gene. Cultured skin fibroblasts from the boy contain an abnormal form of CFTCR protein bound to ubiquitin. Which of the following structures is the most likely site of elimination of these abnormal proteins in this patient? A) Endoplasmic reticulum B) Endosome C) Golgi complex D) Lysosome E) ProteasomeFor each of the following situations, provide a plausible explanation for how it could lead to unrestricted cell division.(a) Colon cancer cells often contain mutations in the gene encoding the prostaglandin E2 receptor. PGE2 is a growth factor required for the division of cells in the gastrointestinal tract.(b) Kaposi sarcoma, a common tumor in people with untreated AIDS, is caused by a virus carrying a gene for a protein similar to the chemokine receptors CXCR1 and CXCR2. Chemokines are cell-specific growth factors.(c) Adenovirus, a tumor virus, carries a gene for the protein E1A, which binds to the retinoblastoma protein, pRb. (d) An important feature of many oncogenes and tumor suppressor genes is their celltype specificity. For example, mutations in the PGE2 receptor are not typically found in lung tumors. Explain this observation. (Note that PGE2 acts through a GPCR in the plasma membrane.)The protein fragments ABS1 and ABS2 of tropomodulin were produced as fusion proteins with chitin binding domain and purified by a chitin column. Explain the principles of this type of affinity chromatography and support it with a self‐drawn figure. This is with regards to Tropomodulin/ F-Actin complex.
- A recent genome sequencing project for the bacterium Burkholderia mallei has identified a new protein with high similarity to the lysylphosphatidylglycerol flippase enzyme. A short section of the new protein sequence is shown below. TVEVNAPGDVQKALSELQQINDGRLDIRI (a) Are any reverse turns likely to be present? Explain your answer. (b) Are any beta-strands likely to be present? Explain your answer. (c) Are any alpha helices likely to be present? Explain your answer. (d) Is any supersecondary structure likely to be present? Explain your answer. (e) Identify two residues that are likely to be buried in the core of the folded protein. Explain your answer. (f) Identify two residues that are likely to be hydrogen bonded to each other. Explain your answer.One single polypeptide chain (120 amino acid residues) is produced for protein A in prokaryotic cell. N-terminal amino acid is alanine in the chain of this protein. How many moles of ATP and GTP will be in use for this polypeptide chain synthesis? What post-translational modifications may be for this protein?The steroid progesterone has an important role in the female reproductive system. Researchers interested in studying membrane progestin receptors (MPRS) developed a method to produce and purify the protein in active form. First, the researchers devised a way to prepare a specific MPR known as hMPRA using the machinery of yeast cells. In order to facilitate purification and identification in later studies, they manipulated the yeast cells so that they attached two different tags to the C-terminal end of the protein. The first tag, Compound 1, is a peptide sequence that acts as an epitope, part of a much larger peptide sequence that is recognized by the immune system. The second sequence consisted of six consecutive histidine residues (His). This sequence binds tightly to Ni2+ cations. In chromatography, (His), tag labeled proteins can be eluted from Ni²+. supported columns by adding a small molecule to the eluent that mimics the side chain of histidine.…
- You are studying a eukaryotic protein PKK in the lab. You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the PKK gene. You also create the following GFP-PKK variants -GFP Wild- type: N- Signal sequence Transmembrane domain Kinase domain Variant 1: N- GFP Transmembrane domain Kinase domain Variant 2: N- -GFP Signal sequence Transmembrane domain Variant 3: N- -GFP Signal sequence Kinase domain Variant 4: N- -GFP Kinase domain If you examine wild-type PKK trafficking in a eukaryotic cell, which of the following are true? Select all that apply This fusion protein will be trafficked to the nucleus This fusion protein is a cell membrane protein This fusion protein will be trafficked to the Endoplasmic Reticulum This fusion protein is a nuclear proteinThe steroid progesterone has an important role in the female reproductive system. Researchers interested in studying membrane progestin receptors (MPRs) developed a method to produce and purify the protein in active form. First, the researchers devised a way to prepare a specific MPR known as hMPRA using the machinery of yeast cells. In order to facilitate purification and identification in later studies, they manipulated the yeast cells so that they attached two different tags to the C-terminal end of the protein. The first tag, Compound 1, is a peptide sequence that acts as an epitope, part of a much larger peptide sequence that is recognized by the immune system. The second sequence consisted of six consecutive histidine residues (His). This sequence binds tightly to Ni2+ cations. In chromatography, (His), tag labeled proteins can be eluted from Ni²+- supported columns by adding a small molecule to the eluent that mimics the side chain of histidine. After preparing hMPRA, the…You are studying a eukaryotic protein PKK in the lab. You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the PKK gene. You also create the following GFP-PKK variants Wild- type: N- -GFP Signal sequence Transmembrane domain Kinase domain Variant 1: N- -GFP Transmembrane domain Kinase domain Variant 2: N- GFP Signal sequence Transmembrane domain Variant 3: N- GFP Signal sequence Kinase domain Variant 4: -GFP Kinase domain If you examine PKK variant 4 trafficking in a eukaryotic cell, which of the following are true? Select all that apply This fusion protein will be made as a cytosolic protein This fusion protein will not function like the wild-type protein This fusion protein will traffic to the cell membrane This fusion protein will not traffic to the cell membrane