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Jisoo was tasked to ensure that an enzyme responsible for keeping her species from extinctior remains biologically active. To do so, she needs to store it in a buffer solution with pH 10.00 Unfortunately, only three acids with their corresponding conjugate base salts are available in thei laboratory as shown below. Ка Conjugate Base Salt Benzoic acid, CsHsCOOH 6.25 x 105 NACSHSCOO Boric acid, H3BO3 5.81 x 107 NaH2BO3 Hydrogen cyanide, HCN 6.20 X 10°10 NACN 1. In what buffer system will Jisoo store the enzyme? 2. Write the dissociation reaction involved in the buffer system. 3. Write the pertinent chemical equations to illustrate what happens upon the addition of smal amounts of (a) strong base and (b) strong acid. 4. Calculate the relative amounts of the buffer components if you were to prepare 250 mL of a 90 mM buffer pH 10.00 using 100 mM stock solutions of its components.

Jisoo was tasked to ensure that an enzyme responsible for keeping her species from extinctior remains biologically active. To do so, she needs to store it in a buffer solution with pH 10.00 Unfortunately, only three acids with their corresponding conjugate base salts are available in thei laboratory as shown below. Ка Conjugate Base Salt Benzoic acid, CsHsCOOH 6.25 x 105 NACSHSCOO Boric acid, H3BO3 5.81 x 107 NaH2BO3 Hydrogen cyanide, HCN 6.20 X 10°10 NACN 1. In what buffer system will Jisoo store the enzyme? 2. Write the dissociation reaction involved in the buffer system. 3. Write the pertinent chemical equations to illustrate what happens upon the addition of smal amounts of (a) strong base and (b) strong acid. 4. Calculate the relative amounts of the buffer components if you were to prepare 250 mL of a 90 mM buffer pH 10.00 using 100 mM stock solutions of its components.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Jisoo was tasked to ensure that an enzyme responsible for keeping her species from extinction remains biologically active. To do so, she needs to store it in a buffer solution with pH 10.00. Unfortunately, only three acids with their corresponding conjugate base salts are available in their laboratory as shown below. Ка Conjugate Base Salt Benzoic acid, CsH;COOH 6.25 x 105 NaCsHsCOO Boric acid, H3BO3 5.81 x 107 NaH2BO3 Hydrogen cyanide, HCN 6.20 X 1010 NaCN 1. In what buffer system will Jisoo store the enzyme? 2. Write the dissociation reaction involved in the buffer system. 3. Write the pertinent chemical equations to illustrate what happens upon the addition of small amounts of (a) strong base and (b) strong acid. 4. Calculate the relative amounts of the buffer components if you were to prepare 250 mL of a 90 mM buffer pH 10.00 using 100 mM stock solutions of its components.
Transcribed Image Text:Jisoo was tasked to ensure that an enzyme responsible for keeping her species from extinction remains biologically active. To do so, she needs to store it in a buffer solution with pH 10.00. Unfortunately, only three acids with their corresponding conjugate base salts are available in their laboratory as shown below. Ка Conjugate Base Salt Benzoic acid, CsH;COOH 6.25 x 105 NaCsHsCOO Boric acid, H3BO3 5.81 x 107 NaH2BO3 Hydrogen cyanide, HCN 6.20 X 1010 NaCN 1. In what buffer system will Jisoo store the enzyme? 2. Write the dissociation reaction involved in the buffer system. 3. Write the pertinent chemical equations to illustrate what happens upon the addition of small amounts of (a) strong base and (b) strong acid. 4. Calculate the relative amounts of the buffer components if you were to prepare 250 mL of a 90 mM buffer pH 10.00 using 100 mM stock solutions of its components.
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