Initiation of transcription in prokaryotic cells requires not only RNA polymerase, but also an "initiation factor" called the sigma factor or sigma subunit. Why can't the core RNA polymerase, without its sigma subunit, initiate transcription?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
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Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Initiation of transcription in prokaryotic cells requires not only RNA polymerase, but also an
"initiation factor" called the sigma factor or sigma subunit. Why can't the core RNA polymerase,
without its sigma subunit, initiate transcription?
The sites of transcription on the DNA has three main regions viz., promoter region (upstream of the gene sequence, helps in initiation of transcription), the gene sequence (the segment of DNA consisting of coding strand and template strand) and a termination sequence. (downstream of the gene sequence, helps in termination of transcription).
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