In this chapter, we described co-immunoprecipitation as a method for identifying binding partners to a protein of interest. Asimpler variation of this method can also be used to isolate proteins of low abundance in a complex mixture.Arrange the steps in sequential order to use this technique for this purpose.First stepLast stepAnswer BankThe protein mixture is centrifuged, the supernatant is removed, and the pellet is washed.The antibody binds to the protein of interest. The antibody-protein complexes become insoluble.An antibody against the protein of interest is added to the mixture.Protein A beads are added to the mixture.

Co-immunoprecipitation (Co-IP) is a laboratory technique used to identify and characterize…

Answered: In this chapter, we described…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Human Immunodeficiency Virus (HIV) Principle: The assay starts with a sample applied to the sample well. A recombinant HIV antigen conjugated to colloidal gold embedded in the sample pad reacts with the HIV antibody present in serum or plasma forming conjugate / HIV antibody complex. As the mixture is allowed to migrate along the test strip, the conjugate / HIV antibody complex is captured by recombinant HIV antigen immobilized on a membrane forming a colored test band in the test region. A negative sample does not produce a test band due to the absence of colloidal gold conjugate / HIV antibody complex. The antigens used in the conjugate test are recombinant proteins that correspond to highly immunoreactive regions of HIV 1 and HIV 2. A colored control band in the control region appears at the end of test procedure regardless of test result. This control band is the result of colloidal gold conjugate binding to the anti-HIV antibody immobilized on the membrane. The…
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