In the presence of saturating amounts of oxaloacetate, the activity of citrate synthase from pig heart tissue shows a sigmoid dependence on the concentration of acetyl-CoA. When succinyl-CoA is added, the curve shifts to the right and the sigmoid dependence is more pronounced. Choose the statements that are reasonable explanations for the right-ward shift of the velocity curve caused by succinyl-CoA. Succinyl-CoA competes with oxaloacetate for binding at the active site. Succinyl-CoA competes with acetyl-CoA for binding at the active site. Succinyl-CoA binds at a regulatory site other than the active site. Succinyl-CoA binds covalently to the enzyme. Succinyl-CoA competes with dissociation of CoA as a product of the reaction. Activity (% of Vmax) 100F No 80- 60- 40 succinyl-CoA I added 20 Succinyl-CoA L 1 L 20 40 60 80 100 120 [Acetyl-CoA] (μm)
Electron Transport Chain
The electron transport chain, also known as the electron transport system, is a group of proteins that transfer electrons through a membrane within mitochondria to create a gradient of protons that drives adenosine triphosphate (ATP)synthesis. The cell uses ATP as an energy source for metabolic processes and cellular functions. ETC involves series of reactions that convert redox energy from NADH (nicotinamide adenine dinucleotide (NAD) + hydrogen (H)) and FADH2(flavin adenine dinucleotide (FAD)) oxidation into proton-motive force(PMF), which is then used to synthesize ATP through conformational changes in the ATP synthase complex, a process known as oxidative phosphorylation.
Metabolism
Picture a campfire. It keeps the body warm on a cold night and provides light. To ensure that the fire keeps burning, fuel needs to be added(pieces of wood in this case). When a small piece is added, the fire burns bright for a bit and then dies down unless more wood is added. But, if too many pieces are placed at a time, the fire escalates and burns for a longer time, without actually burning away all the pieces that have been added. Many of them, especially the larger chunks or damp pieces, remain unburnt.
Cellular Respiration
Cellular respiration is the cellular process involved in the generation of adenosine triphosphate (ATP) molecules from the organic nutritional source obtained from the diet. It is a universal process observed in all types of life forms. The glucose (chemical formula C6H12O6) molecules are the preferred raw material for cell respiration as it possesses a simple structure and is highly efficient in nature.
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