In the Isoelectric Precipitation of Proteins: Casein from Milk - What would happen to the precipitation reaction if 0.2N H2SO4 is added instead of 0.2N HCl
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In the Isoelectric Precipitation of Proteins: Casein from Milk - What would happen to the precipitation reaction if 0.2N H2SO4 is added instead of 0.2N HCl
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- Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL X80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is then titrated with 0.2M HCL to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCL needed to reach relevant pKa value and equivalence point(s)z Relevant pKa values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.
- What volume of 0.1M NaOH (reagent) needs to be added to increase your 7.5 pH (buffer) by 0.5 pH units. The goal is to further purify MOPs (see image). Protonated form = 6.6 x 10.3^-3 mol/L Deprotonated form = 1.32 x 10^-2 mol/L Show calculations.Quinine ( C20 H24 N2 O2) is the most important alkaloid derived from cinchona bark. It is used as an antimalarial drug. For quinine, pK, = 5.1 and pK, = 9.7 ( pKp = – log Kp). Only 1 g quinine will dissolve in 1920.0 mL of solution. Calculate the pH of a saturated aqueous solution of quinine. Consider only the reaction | Q+ H2O= QH+ + OH- described by pK, where Q = quinine. pH =You need to make a protein buffer of: . • • 100 mM NaCl 25 mM Tris 8 5% w/v glycerol • 2 mM DTT Your stock solutions are: 5 M NaCl • 2 M Tris 8 • 70% w/v glycerol • DTT @ 154.25 g/mol How would you make a 1L protein buffer Solution? Show your work and describe.
- The isoelectric point of eIF4a is 5.02. Students are given a sample of cell lysate containing eIF4A at pH 7.4. They are also provided with two buffers of pH 7.4 to use for ion exchange chromatography. One buffer has a very high salt concentration (1 M), the other has a low salt concentration (0.1 M). Mariela decides to use an anion exchange column, while Ashok chooses a cation exchange column. Who made the better decision? Provide a detailed explanation of why one student will end up with a purer sample of eIF4A. Which buffer will you use while washing away impurities (high or low salt), and which would you use to remove eIF4A from the column? Explain your choice.A patient presents to the emergency department in a critical condition. The patient’s blood was tested and the concentration of carbonic acid (H2CO3) was found to be 4.0×10-4 M and bicarbonate ion (HCO3-) 3.062×10-3 M. Ka = 4.3 × 10-7 H2CO3(aq) ⇌ HCO3-(aq) + H+(aq) Show all calculations including equation(s) used. Calculate the pH of the patients’ blood. Based on your answer would you say the patient is suffering from Acidosis or Alkalosis?You are asked to prepare HEPES buffered saline with the following constituents: НЕРES 10mM KCI 5mM NaCl 135mM CaCl2 1mM M9SO4 0.6mM Glucose 6mM You have previously made up 100 mM stock solutions of HEPES, KCI, CaCl2, and M9SO4 and a 1M stock of NaCl. The MW of glucose is 180.16 g/mol What volume of HEPES would you require to make up 400ml of buffer?
- N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) buffer at pH 7 with 400 mM NaCl. A A purified protein is in a Hepes dialysis membrane tube holds a 5.0 mL sample of the protein solution. The sample tube floats in a beaker containing 0.50 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na*, CI", and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 5.0 mL protein sample after dialysis in 300 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: mM mMThe pl of alkaline phosphatase is 4.5; the pl of the DEAE cellulose is 10.5. We used a buffer of pH 7.4 to run a DEAE-cellulose as anion exchange column? Explain how did this work?Calculate the concentration of Y4-ion in 0.0100 M EDTA solution at pH 6. α4 = 2.2 x 10-5 for pH 6.