In the biology laboratory mixtures of chemicals (called reagents) are often used. These also have MSDS. Study MSDS for these biological reagents: 1. Benedict's Solution- used for detecting sugars 2. Biuret Solution - used for detecting proteins 3. Lugol's Solution - used for detecting starches What chemicals do they contain? Benedict's Solution abholdo Biuret Solution Lugol's Solution What personal protective equipment is recommended to avoid the health or contact hazard?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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**Exercise 2**

In the biology laboratory, mixtures of chemicals (called reagents) are often used. These also have MSDS (Material Safety Data Sheets). Study MSDS for these biological reagents:

1. Benedict’s Solution – used for detecting sugars
2. Biuret Solution – used for detecting proteins
3. Lugol’s Solution – used for detecting starches

**What chemicals do they contain?**

| **Benedict’s Solution** |                                              |
|--------------------------|----------------------------------------------|
| **Biuret Solution**      |                                              |
| **Lugol’s Solution**     |                                              |

**What personal protective equipment is recommended to avoid the health or contact hazard?**
Transcribed Image Text:**Exercise 2** In the biology laboratory, mixtures of chemicals (called reagents) are often used. These also have MSDS (Material Safety Data Sheets). Study MSDS for these biological reagents: 1. Benedict’s Solution – used for detecting sugars 2. Biuret Solution – used for detecting proteins 3. Lugol’s Solution – used for detecting starches **What chemicals do they contain?** | **Benedict’s Solution** | | |--------------------------|----------------------------------------------| | **Biuret Solution** | | | **Lugol’s Solution** | | **What personal protective equipment is recommended to avoid the health or contact hazard?**
### Use of LD50 to Evaluate Toxicity

The LD50 of a substance is the amount of a material that will kill 50% of the organisms (usually rats or mice) after one dose.

Using the internet, look up the oral LD50 of:

| **SUBSTANCE**         | **LD50** |
| -------------------------- | ----------- |
| Table salt (NaCl)        |                  |
| Caffeine                      |                  |
| Ricin                            |                  |
| Ethanol                       |                  |

**Which is the most toxic? How do you know?**

### Case Study

A mother saw her son John eating aspirin pills from an open bottle. The little boy weighed 22 pounds. Calculate the number of aspirin tablets that would be an LD50 dose for this child.

- The oral LD50 of aspirin is 200mg/kg.
- 1 kg equals 2.2 pounds.

**Questions:**
1. What is John’s weight in kg?
2. What is the LD50 of aspirin for John’s weight?
3. One aspirin tablet is equal to 325 mg. How many aspirin tablets are required to reach that LD50 quantity? __________

**Discussion:** Are you in favor of child safety tops on pill bottles?
Transcribed Image Text:### Use of LD50 to Evaluate Toxicity The LD50 of a substance is the amount of a material that will kill 50% of the organisms (usually rats or mice) after one dose. Using the internet, look up the oral LD50 of: | **SUBSTANCE** | **LD50** | | -------------------------- | ----------- | | Table salt (NaCl) | | | Caffeine | | | Ricin | | | Ethanol | | **Which is the most toxic? How do you know?** ### Case Study A mother saw her son John eating aspirin pills from an open bottle. The little boy weighed 22 pounds. Calculate the number of aspirin tablets that would be an LD50 dose for this child. - The oral LD50 of aspirin is 200mg/kg. - 1 kg equals 2.2 pounds. **Questions:** 1. What is John’s weight in kg? 2. What is the LD50 of aspirin for John’s weight? 3. One aspirin tablet is equal to 325 mg. How many aspirin tablets are required to reach that LD50 quantity? __________ **Discussion:** Are you in favor of child safety tops on pill bottles?
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