In table 21.1, the effects of injection of different types of RNA into wild type mice was examined. Explain why injection of the the miRNA to the Kit mRNA resulted in white feet and tails, but an injection of a non-specific mRNA had no effect.

Q: Northern blots are valuable tools to analyze the mRNA level present in a sample. Describe an…

A: The goal of a northern blot is to monitor the expression of a particular genome in tissues, organs,…

Q: A particular mature miRNA (microRNA) in mice has sequence 5'-UUCCCUGAGACCUCAAGUGUGA-3'. a. If you…

A: Answer a) ii and iii ii) mRNA sequence complementary to the miRNA sequence iii) miRNA sequence with…

Q: Match each of the following descriptions to the type of regulatory RNA affecting a "target" gene…

A: Of course! Let's break down each match:This regulatory RNA may increase transcription of the target…

Q: Give the result(s) of the experiment in "MicroRNAs Control De Novo DNA Methylation Through…

A: INTRODUCTION MicroRNAs (miRNAs) are a class of small, single-stranded non-coding RNAs that regulate…

Q: Compare and contrast the properties and roles of siRNAs and miRNAs in RNA interference. Be sure to…

A: RNA interference (RNAi) is a gene regulation mechanism that functions by suppressing the expression…

Q: Explain why knowing the orientation of a promoter is critical when determining the amino acid…

A: Promoters are nucleotide sequences of deoxyribonucleic acid that potentially modify binding of RNA…

Q: MicroRNA (miRNA) in eukaryotes are similar to anti-sense RNA in bacteria in that they regulate…

A: RNA is one of the three principal biological macromolecules required for all well-known life forms…

Q: A shortened version (apo B-48) of apolipoprotein B is formed by the intestine, whereas the…

A: Chylomicrons are a type of lipoprotein particles made up of phospholipids, cholesterol, proteins,…

Q: Explain the reason why different parts of the mRNA is represented at different levels in an…

A: Introduction: Alternative splicing, sometimes referred to as differential splicing, is a unique…

Q: You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin…

A: Restriction enzymes are use to cut DNA at specific sites, known as restriction sites. The…

Q: doctoral student in biology, Esther Mensah, is reviewing primary literature over RNAi interference…

A: AML gene I.e., Acute Myeloid Leukemia are the cancerous cells which might be the result mutations…

Q: Northern blotting depends on the phenomenon of the binding of aprobe to mRNA. In this technique,…

A: Northern Blotting is a blotting technique used to detect the presence of a specific ribonucleic acid…

Q: true/ false Drosha and Pasha bind to mRNAs allowing the 43S subunit to form, but they inhibit the…

A: Drosha is a type III RNase, which plays a critical role in miRNA biogenesis. Pasha is an essential…

Q: One of your patients, a six-year-old girl who suffers from Sickle cell anemia, an inherited blood…

A: Answer to the question :  C)  translation,  on ribosome.

Q: miRNAs target endogenous mRNAs in a sequence-specific manner. Explain, conceptually, how one might…

A: To explain: How to identify potential mRNA targets for a given miRNA if only the sequence of the…

Q: Explain how the formation of one stem–loop in the RNA canblock the formation of another.

A: Bacteria are genetically regulated by RNA, they use a ribonucleic acid sequence encoded within mRNA…

Q: Describe the recognition process by which the tRNA for Nformylmethionine interacts with the portion…

A: N-formylmethionine is a derivative of the amino acid methionine in which a formyl group has been…

Q: The pre-initiation complex recognizes the EIF4 group factors on the mRNA (EIF4E at 5' cap and EIF4G…

A: The translation is the step of central dogma where the information of life stored in DNA  is…

Q: AU-rich elements (AREs) are cis-elements in mRNAs that regulate stability and decay. How is it…

A: The post-transcriptional gene expression is regulated by the cis-acting regulatory sequence elements…

Q: RNA splicing

A: Most eukaryotic cells undergo post-transcriptional modification, which involves chemically altering…

Q: In bacteria, genes that are often used together are controlled by a single promoter. Explain why…

A: Gene regulation refers to the numerous processes that regulate which genes are expressed and the…

Q: In Figure 8-6, describe where the gene promoter islocated.

A: DNA refers to a polymer of nucleotides and is double-stranded. One of the strands of DNA plays a…

Q: Some eukaryotic mRNAs contain an AU-rich element in the 3’ untranslated region.  What would be the…

A: The regulation of gene expression is done by the pre-mRNA processing and degradation of mRNA. The…

Q: What is miRNA replacement therapy? Describe three examples of this treatment approach.

A: A Gene is a unit of heredity containing the fundamental information of life as a distinct sequence…

Q: In bacteria, a transcription experiment in vitro with purified RNA polymerase holoenzyme is fairly…

A: Bacteria are one of the type of biological cell. They constitute a large domain of the  prokaryotic…

Q: The presence of the MCS in the coding region also results in us being able to express inserts…

A: The Multiple Cloning Site (MCS) is a short segment of DNA containing many (multiple) restriction…

Q: While miRNA response elements (MREs) may be located anywhere within an mRNA, they are most often…

A: Micro-RNAs are defined as a class or group of non-coding RNAs that will play an essential role in…

Q: You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin…

A: The expression level of any expression vector depends on the regulatory elements like enhancer and…

Q: ould be a possible result if a essed in the cell?

A:

Q: A doctoral student in biology, Esther Mensah, is reviewing primary literature over RNAi interference…

A: Gene expression describes the transformation of genetic information contained in a gene into a…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.