in response to this question, could you explain why in Step 3, R became 0.500 and why in Step 4, Vmax is sudden multiplied by 10^-3? It's unclear in the response 10:25 AM Wed Mar 9A 59%b Answered: Determine the X+a bartleby.comGet live help whenever you need from online tutors! Try bartleby tutor today →bartlebyISSEARCH& ASK AN EXPERTCHATVx MATH SOLVERScience » Chemistry » Q&A Library » Determine the values of KM and Vmax. For the Vmax obtained, calculate the turnover num...Question• Determine the values of Ky and Vmax. For the V,obtained, calculate the turnover number assumingтах*таxthat 1X 104mol of enzyme were used.Substrate concentration (mol L²)Velocity (mM min 1)2.5000.5881.0000.5000.7140.4170.5260.3700.2500.256Expert SolutionStep 1The graph formed will be as follows:Graph of Velocity v/s Substrateconcentration0.70.60.50.40.30.2Velocity (R) 10:26 AM Wed Mar 9A 59%b Answered: Determine the X+a bartleby.comGet live help whenever you need from online tutors! Try bartleby tutor today →Q SEARCH& ASK AN EXPERT보 CHATVx MATH SOLVERStep 3Putting the value of vmax in equation (1):V.max[S]R =[S]+Km0.666 mM min×(1×10³) mM(1×10³) mM +Km0.500 mM min--3K = 0.332×10 mM%3DmStep 4Turn over number can be calculated as follows:V.max= k,[E,](3)....where E, is the enzyme concentration, k2 is the turn over number, vmax is the maximum rate. Assumingthat all of the enzyme has bind, k2 can be calculated by putting the values in equation (3) as follows:0.666 ×10³M min = k, ×1×10ª M-1k, = 6.66 min

Michaelis menten constant, Km is the substrate concentration required to produce half maximum…

Answered: in response to this question, could you…

in response to this question, could you explain why in Step 3, R became 0.500 and why in Step 4, Vmax is sudden multiplied by 10^-3? It's unclear in the response

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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