In ion-exclusion chromatography, ions are separated from nonelectrolytes (uncharged molecules) by an ion-exchange column. Nonelectrolytes penetrate the stationary phase, whereas ions with charge of the same sign as that of the stationary phase are repelled by the stationary phase. Because electrolytes have access to less of the column volume, they are eluted before nonelectrolytes. A mixture of trichloroacetic acid (TCA, pKa 5 20.5), dichloroacetic acid (DCA, pKa 5 1.1), and monochloroacetic acid (MCA, pKa 5 2.86) was separated by passage through a cation-exchange resin eluted with 0.01 M HCl. The order of elution was TCA , DCA , MCA. Explain why the three acids are separated and the order of elution.
In ion-exclusion chromatography, ions are separated from nonelectrolytes (uncharged molecules) by an ion-exchange column. Nonelectrolytes penetrate the stationary phase, whereas ions with charge of the same sign as that of the stationary phase are repelled by the stationary phase. Because electrolytes have access to less of the column volume, they are eluted before nonelectrolytes. A mixture of trichloroacetic acid (TCA, pKa 5 20.5), dichloroacetic acid (DCA, pKa 5 1.1), and monochloroacetic acid (MCA, pKa 5 2.86) was separated by passage through a cation-exchange resin eluted with 0.01 M HCl. The order of elution was TCA , DCA , MCA. Explain why the three acids are separated and the order of elution.
Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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In ion-exclusion chromatography, ions are separated from nonelectrolytes (uncharged molecules) by an ion-exchange column. Nonelectrolytes penetrate the stationary phase, whereas ions with charge of the same sign as that of the stationary phase are repelled by the stationary phase. Because electrolytes have access to less of the column volume, they are eluted before nonelectrolytes. A mixture of trichloroacetic acid (TCA, pKa 5 20.5), dichloroacetic acid (DCA, pKa 5 1.1), and monochloroacetic acid (MCA, pKa 5 2.86) was separated by passage through a cation-exchange resin eluted with 0.01 M HCl. The order of elution was TCA , DCA , MCA. Explain why the three acids are separated and the order of elution.
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