In cell counting, why do you need to calculate for the volume of media needed after computing the cells

Q: Density gradient centrifugation is an inexpensive cell separation technique. Mention 2 limitations…

A: Density gradient centrifugation is a common technique for isolating and purifying biomolecules and…

Q: did water flow into or out of the plant cells (potato cells) in each of the samples examined? How do…

A: Osmosis is the movement of water molecules or solvent molecules from its high concentration towards…

Q: Why does counting 5 Boxes in each chamber of the hemacytometer gives you the number of cells in a ul…

A: Hemacytometer is a medical instrument used to measure the concentration of blood components in a…

Q: There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one…

A: Let's say, the yeast cell culture which was incubated for 6  hours as  = sample A the yeast cell…

Q: In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are…

A: Given,number of colonies in a 1mL of culture = 250. Also,given that this given solution is 100 times…

Q: What are the key factors that affect the success of cell culture techniques in a laboratory setting?

A: Choosing an appropriate cell line for the intended purpose is essential. Factors to consider include…

Q: You want to add cells at a density of 3 x 104 cells / cm2 to a 12-well plate where each well has an…

A: Cell density:It is very tightly regulated and varies very little among given cell type. Changes in…

Q: Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will…

A: Cell fractionation is a process that involves rupturing cells, separating and suspending cellular…

Q: the cell potential table found in the Reference Material,  why do you get holes in your aluminum…

A: The natural process that results in the conversion of a reactive metal into a stable metal by…

Q: Steven is a lab research assistant. He mixed 25 µL of cells with 50 µL of cell culture media in…

A: A Hemocytometer slide is a specially designed glass slide with Neubar's rulings in the central…

Q: How would I figure out how many dilutions to make given cell density ? And volume to plate ? For…

A: Serial dilutions (log dilutions) are used to reduce bacterial concentrations to the levels necessary…

Q: You are observing a cell in the microscope at 100X and determine that it has a length of 15 ocular…

A: The question should also have mentioned the micrometer lens data. But because it is not mentioned…

Q: The Leica microscope that we will be using for histology lab has four different objective lenses and…

A: Microscopes are used to magnify an object. In biology laboratories compound light microscopes are…

Q: What conclusion would you draw if the numbers of bacterial colonies in were the same on the control…

A: Bacteria are unicellular and prokaryotic organisms that live in wide variety of environments. It…

Q: Assume you used a hemocytometer to count your cells. Given the results below, calculate the total…

A: A hemocytometer is a specialised counting chamber used in laboratories to count and gauge the…

Q: If 100,000 normal human cells were plated in a petri dish, around how many normal cells would be…

A: Introduction: If we start with one cell, when it divides, there are 2 cells in the first generation,…

Q: Carrot slices placed in 0.2M salt solution for several hours becomes flaccid (limp). Carrot slices…

A: Tonicity is the relative concentration of solutes dissolved in solution which determine the…

Q: What is the purpose of osmosis lab?

A: Osmosis is defined as..

Q: The size of a cell seen under a microscope is 3mm Using a total magnification of 40x. What is the…

A: The formula to calculate the actual size of the cell is given below.   Actual size of the cell =…

Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…

A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…

Q: Compare two types of membrane transport proteins by completing the blanks below. a) ATP Needed or…

A: Carrier proteins are proteins that carry substances from one side of a biological membrane to the…

Q: You have are diluting down a concentrated stock of bacteria in order to grow them. Starting with 2.1…

A: A thick solution can be made into a more useful concentration by performing a series of sequential…

Q: If 10 µL of cells in suspension was mixed with 20 µL of a dye called trypan blue. DF = _____.…

A: According to the question, If 10 µL of cells in suspension was mixed with 20 µL of a dye called…

Q: What cell types would be able to grow on the ECM in the following situations? a) Streptomycin was…

A: The extracellular matrix (ECM) is a complex network of proteins and carbohydrates that provides…

Q: What do the following abbreviations mean: DdDp, RdDp, DdRp, RdRp. Which could be provided by cells?

A: Answer: Introduction: RNA-dependent RNA polymerase (RdRp) is also called as RNA replicase, it is a…

Q: You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell…

A: Hemocytometer is device used for the counting of blood cells in a sample. It has counting chambers…

Q: a) Make a drawing of the cells from each culture to identify the various stages of the cell…

A: Saccharomyces cerevisiae is a eukaryotic organism that is used in various scientific experiments. It…

Q: What diseases and disorders will affect the regulations of liquid liquid phase separation in cells…

A: Our cells rely on a delicate dance called liquid-liquid phase separation (LLPS) to organize…

Q: You are testing a potential new antibiotic for its effectiveness at killing Streptococcus…

A: Drugs are medications that are taken in the prescribed amounts under the supervision of doctors to…

Q: My disinfectant says it will kill 99.99% of bacteria. The directions say to achieve this kill rate I…

A: Sterilization is a physical or chemical process of eradicating harmful living microbes. These…

Q: Where can we practically use the knowledge acquired in knowing the differences of Hypertonic,…

A: Introduction A solution is made up of solvent and solute. The solvent is usually in aqueous form and…

Q: n this part of the assignment, assume you have carried out the microscopic examination of the…

A: Depending on the amount of solute and solvent present, a solution is classified into three…

Q: determine if osmosis or simple diffusion

A: The main criteria that will be used in this particular question will be the movement of molecules…

Q: What is the size in µm of a cell that is 135mm wide in my photo? The scale bar in the photo is…

A: A microscope is a scientific instrument that allows us to see objects that are too small to be seen…

Q: Describe a technique that will allow you to separate cells from media

A: ANSWER) There are various techniques which are used to separate the cells from the medium. Some of…

Q: CELL COUNTING FOR DETERMINING VIABLE CELL CONCENTRATION Please give me a general introduction to…

A: The smallest structural and functional unit of life is the cell. It's just a piece of rudimentary…

Q: draw the results of a flow cytometry experiment in which one set of cells is treated with vehicle…

A: Taxol is a microtubule-stabilizing drug commonly utilized to treat cancer and can actuate cell cycle…

Q: Which cell represents a hypotonic solution?

A: Answer: OSMOSIS : It is the movement of water from the higher concentration to the lower…

Q: If a cell sample is placed in pure water, what is the concentration of the salt inside the cell?…

A: The water move inside the cell and outside the cell through the process of osmosis. If a cell is put…

Q: The cell is usually placed after the monochromator, except in the case of using a multichannel…

A: A multi-channel analyzer is an instrument used in laboratory and field applications so to analyze an…

Q: nna is training to be a cell culture technician. She uses some sterile distilled water to wasg a…

A: The amount of dissolved solute per unit volume is referred to as osmotic concentration. Based on…

Q: A stock solution of a bacterial strain is diluted 1/10000 to a total volume of 500ul. Then, 50ul of…

A: The mixing of the stock solution with the dilutant liquid is known as dilution. The ratio of stock…

Q: You wish to identify the presence of a specific protein inside the cell. What microscopy technique…

A: Microscopy is a scientific technique that involves the use of microscopes to observe and study…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.