Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
in a western blot how come changes in mrna levels don't always match the protein product of that mRNA.
The western blot:
It is also known as the protein immunoblot. It is an extensively used analytical method in the immunogenetics, molecular biology, and other biology disciplines to distinguish the precise proteins in a sample of tissue extract or homogenate.
Process:
SDS-polyacrylamide gel electrophoresis is used in this method. It is used to separate the different proteins present in the given sample.
The distinguished proteins are then transferred onto a matrix. There these are stained with antibodies precise to the target protein. By knowing the site and intensity of the definite reaction, expression details of the selected proteins of the given tissue homogenate could be acquired.
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