In a sample dsDNA from an organism, the amount of thymine is analyzed to be 12 μmoles when the A+T/G+C ratio is 6.0. How much cytosine should be in the sample? 3 3.5 4 2
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In a sample dsDNA from an organism, the amount of thymine is analyzed to be 12 μmoles when the A+T/G+C ratio is 6.0. How much cytosine should be in the sample?
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- At 1x concentration, the orange G dye has 5% glycerol. The dense glycerol will help keep your DNA in your samples from floating out of the wells upon loading the gel. What is the concentration of glycerol in the 6x orange G stock solution? Be sure to show your calculations.In a sample dsDNA from an organism, the amount of thymine is analyzed to be 12 umoles when the A+T/G+C ratio is 6.0. How much cytosine should be in the sample? 3.5 2 3A 10 μL-aliquot of a resuspended genomic DNA stock solution was obtained and further diluted by adding 990 μL TE buffer. The A260 of the resulting solution was 0.316. Determine its concentration in µg/µL.
- A mixture of five proteins are analyzed by MALDI-TOF. Their molecular weigths are 3 kD, 22 kD, 100 kD, 225 kD and 515 kD. A peak appears in the mass spectrum at 33.3 kD. Which protein most likely gave this peak?You will use a Calf Thymus DNA standard stock solution (1 mg/ml) to prepare a standard curve. Calculate volume of stock solution in ul, needed to prepare 10 ug/ml dilution (final volume 1.0 ml).The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis: Molecular weight (Daltons) Protein Isoelectric point Myoglobin 17,000 7.0 Cytochrome C 12,000 10.6 IgG 150,000 6.4 Hemoglobin 64,500 7.1 Insulin 5,800 5.4 Human serum albumin 66,000 4.6 Histone H3 15,000 10.8 Рepsin 33,000 1.0 In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller proteins go towards the bottom. 2 4 pH 7 10 direction of electric current 150 MW (kDa) 100 В E A 50 2 4 pH 7 10 With this information, match the letters of the bands to the proper protein based on the data given. Band Letter Protein A D E
- Using a 1 cm cuvette, the absorbance at 260nm of your double-stranded DNA sample is 0.15. What is the concentration of your sample?Four proteins Cytochrome C (pI=10.2) Myoglobin (pI=7.2), Hemoglobin (pI = 6.8) and Serum Albumin (pI= 4.8) were used in our gel electrophoresis lab exercise. If the electrophoretic separation is carried out at pH 7.0, which protein would move toward the negative electrode? Cytochrome C Myoglobin HemoglobinOne OD unit of A260 is 50 ug/ml dsDNA. You have 2.75 ml genomic DNA (stock). You took 50 ul of the DNA sample and mixed it with 950 ul water and measured the A260 absorption. The diluted sample absorbed 0.14 units of A260. The DNA concentration of the stock is ug/ml, considering the dilution factor of (Note: ug microgram, ul- microliter). 70/10 140/20 280/50 O 560/100
- Explain how to prepare 2 ml of a solution with a concentration of 1 μg/5ml from a stock solution of a DNA sample with a concentration of 0.1 mg/ml.You have a 20 mg/ml of Ethidium bromide stock solution. You need to a final concentration of 2ug/ml into a Agarose solution to visualize DNA. What is the dilution factor? Give typing answer with explanation and conclusionThe figure shows that the average distance between base pairs measured parallel to the axis of a DNA molecule is 3.4 Å. The average molar mass of a pair of nucleotides is 650 g•mol-1. Estimate the length in cm of a DNA molecule of molar mass 5.1x109 4.0 .26 g•mol-1. cm Roughly how many base pairs are contained in this molecule? [4.0 784615 x base pairs CG G C OH TA Thymine Adenine H. H. OH CH2 N-H.......O CH3 H H N……·H–N HLH TA OH 0-P H2C CG G C H. CH2 Guanine H OH Cytosine ... H-N TA AT H' N.....H-N HLH N-H.......O H2C OH H -o. -P3D0 TA 0- OH H. -CH2 (b) The most common structure of DNA, which is a right-handed double helix. The two strands are held together by hydrogen bonds (a) Base-pair formation between adenine (A) and thymine (T) and between cytosine (C) and guanine (G). and other intermolecular forces.