O Macmillan LearningIn a famous experiment, Matthew Meselson and Franklin Stahl grew E. coli cells in a medium containing only the "heavy"isotope of nitrogen, 15 N. These cells were then transferred to a medium with the "light" isotope of nitrogen, ¹4N. Theresults of the experiment supported the hypothesis of semiconservative replication, which was proposed by Watsonand Crick.Heavy DNA (¹5N DNA), hybrid DNA, and light DNA (¹4N DNA) can be separated by centrifugation. If cells containing15N DNA are transferred to a medium with only ¹4N NH4Cl as a nitrogen source, what percent of daughter molecules arecomposed of hybrid DNA after 3 generations?daughter molecules:%

DNA replication is a process by which each strand of DNA acts as a template and copies itself to…

Answered: In a famous experiment, Matthew…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The following sequences of DNA would be synthesized using 5′-CAGTTCGGA-3′ as a template: * 3′-AGGCTTGAC-4′ 3′-TCCGAACTG-5′ 3′-GTCAAGCCT-5′ 3′-CAGTTCGGA-5′
As shown, five DnaA boxes are found within the origin of replication in E. coli. Take a look at these five sequences carefully. A. Are the sequences of the five DnaA boxes very similar to each other? (Hint: Remember that DNA is double-stranded; think about these sequences in the forward and reverse directions.) B. What is the most common sequence for a DnaA box? In other words, what is the most common base in the first position, second position, and so on until the ninth position? The most common sequence is called the consensus sequence. C. The E. coli chromosome is about 4.6 million bp long. Based on random chance, is it likely that the consensus sequence for a DnaA box occurs elsewhere in the E. coli chromosome? If so, why aren’t there multiple origins of replication in E. coli?
For a science fair project, two students decided to repeat the Hershey and Chase experiment, with modifications. They decided to radioactively label the nitrogen of the DNA, rather than the phosphate. They reasoned that each nucleotide has only one phosphate and two to five nitrogen atoms. What would be the results of this experiment? O Radioactive nitrogen would be found inside the bacterial cells. Radioactive nitrogen would be found in the supernatent/environment and not in the bacterial cells. The experiment wouldn't work because DNA does not contain nitrogens. O Radioactive nitrogen would be found in both the supernatent/environment and the bacterial cells. MacBook Air DII DD 80 F9 000 F7 F8 F5 F6 F3 F4 * &
How many bands of DNA would be expected in Meselson and Stahl’s experiment after two rounds of conservative replication?
There are 6 parts to this question: This is a follow up to the prior question regarding the replication of the DNA strand below. The DNA strand is here for your reference and you do not need to do anything with or to it. TC GATATCGG AGCTATAGCC c) what enzyme separated the parental DNA template strands, d) what bonds were broken? e) what enzyme replicates DNA f) before DNA can be replicated/copied, what must be laid down to allow the enzyme in "e" to replicated the DNA (be specific)? g) our DNA is replicated in many "pieces", what enzyme connects these many "pieces" into one continuous DNA strand that becomes the sister chromatid? h) during what specific phase of the cell cycle does this DNA replication process occur? (This should be a review question from last topics we covered).

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