We are practicing calculating for making solutions. How would I calculate this? If you want to set up an enzyme reaction. You have the following,10X buffer10U/ul enzyme50 ng/ul DNAWater.You want a final 40 ul reaction with 250 ng of DNA, 1x buffer, and 2 units of Enzyme. How much ofeach of the above do you need?

1. DNA Calculation (Most detailed): • Target amount: 250 ng of DNA• Stock concentration: 50 ng/μl•…

Answered: If you want to set up an enzyme…

Introductory Chemistry For Today

8th Edition

ISBN:9781285644561

Author:Seager

Publisher:Seager

Chapter9: Acids, Bases, And Salts

Section: Chapter Questions

Problem 9.142E

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Time to purify biochemisfunase! To do this, you’ll need to make MORE buffers. One of the buffers that you need to make requires 150 mM Tris (pH 8), 250 mM sodium chloride (NaCl) and 2 mM dithiothreitol (DTT, a reducing agent that prevents the formation of disulfide bonds in proteins). Your jolly postdoc friend wants you first to make 500 ml of a1.0M Tris stock solution before making the buffer. Describe how you would make this stock solution, and show all calculations.
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If you want to set up an enzyme reaction. You have the following, 10X buffer 10U/ul enzyme 50 ng/ul DNA Water. You want a final 40 ul reaction with 250 ng of DNA, 1x buffer, and 2 units of Enzyme. How much of each of the above do you need?