We are practicing calculating for making solutions. How would I calculate this? If you want to set up an enzyme reaction. You have the following,10X buffer10U/ul enzyme50 ng/ul DNAWater.You want a final 40 ul reaction with 250 ng of DNA, 1x buffer, and 2 units of Enzyme. How much ofeach of the above do you need?

1. DNA Calculation (Most detailed): • Target amount: 250 ng of DNA• Stock concentration: 50 ng/μl•…

Answered: If you want to set up an enzyme…

Introductory Chemistry For Today

8th Edition

ISBN:9781285644561

Author:Seager

Publisher:Seager

Chapter9: Acids, Bases, And Salts

Section: Chapter Questions

Problem 9.142E

See similar textbooks

Similar questions

Time to purify biochemisfunase! To do this, you’ll need to make MORE buffers. One of the buffers that you need to make requires 150 mM Tris (pH 8), 250 mM sodium chloride (NaCl) and 2 mM dithiothreitol (DTT, a reducing agent that prevents the formation of disulfide bonds in proteins). Describe how you would make 1.5L of the buffer using your newly constructed 1.0 M Tris stock solution, a 500 mM stock solution of NaCl, and a 1.0 M stock solution of DTT. Show all calculations.
5
PLS HELP ASAP ON ALL ASKED QUESTIONS PLS
The K, for acetic acid (CH, COOH) is 1.737 x 10-5. What is the pK, for this acid? pK = Use the calculated pK, to determine the pH at which [CH, COOH] = [CH,COO"]. pH =
An enzyme reaction produces 1.2 x 10-4 M hydrogen ions during the 1.0 min duration of the reaction. The enzyme reaction mixture is buffered by a 0.10 M Tricine (pKa 8.16), pH 8.3. What is the new pH after the reaction is over? *hint* the Henderson-Hasselbalch equation is used twice*
20. Which of the following acid/ conjugate base pairs would function best as a buffer at physiological pH? a Lactic acid / Lactate ion; pKa = 3.86 b Carbonic acid / Bicarbonate ion; pKa = 6.37 c Bicarbonate ion / Carbonate ion; pKa = 10.25 d Dihydrogen phosphate / Monohydrogen phosphate ion; pKa = 6.86 e Acetic acid / Acetate ion; pKa = 4.76
You take 10.00 grams of pure acetic acid add top it up to 1.00 liter of solution. What is the pH of the solution at equilibrium? You will need to look up the pKa or Ka of acetic acid to figure this out (just google it). You add 20.00 grams of sodium acetate to the mixture and it causes a negligible increase in volume. What is the new pH once the solution reaches equilibrium? You add 1.00 mole of HClO4 (a strong acid) causing a negligible increase in volume again. What is the new pH? You add 1.00 mole of NaOH (strong base) causing a negligible increase in volume AGAIN (somehow?). What is the new pH?
Fe3+(aq) + SCN-(aq) to Fe(SCN)2+(aq). Identify the reactant or product that increases or decreases when AqNO3 is added and AgSCN(s) formed.
Good explanation Asap Thanks Hemoglobin, Hb, has four Fe atoms per α molecule that, on average, bind about three O2 molecules. Hb(aq)+3O 2 (g) = Hb(O 2 ) 3 (aq)Discuss mountain or space sickness (high altitude) in terms of this balance.
Lactase is an enzyme that breaks down the sugar lactose which is commonly found in dairy products. Some people develop lactose intolerance as they grow older and the amount of lactase enzyme in their gut diminishes. Since the enzyme is commonly found in the gut of humans it can withstand a pH that is very acidic, ranging from a pH of 2-7. A student decided to do an experiment to determine how the pH of a solution affects lactase enzyme activity. Lactase works by breaking lactose into its two component sugars of glucose and galactose. To conduct the experiment the student prepared a solution of lactose and placed it into 5 different test tubes. A pH buffer solution was then added to each test tube with a pH of 2.0 in the first tube, 4.0 in the second, 6.0 in the third, 8.0 in the fourth, and a 10 pH in the fifth. Next, the enzyme lactase was added in equal amounts to each test tube and allowed to sit for ten minutes white the enzyme reacted with the lactose. At the end of the ten…
Write the Ksp expression for the sparingly soluble compound silver phosphate, Ag3PO4. Ksp = If either the numerator or denominator is 1, please enter 1.
Which of the following acid/ conjugate base pairs would function best as a buffer at physiological pH? Lactic acid / Lactate ion; pka = 3.86 Carbonic acid / Bicarbonate ion; pka = 6.37 Bicarbonate ion / Carbonate ion; pKa = 10.25 Dihydrogen phosphate / Monohydrogen phosphate ion; pKa = 6.86 Acetic acid / Acetate ion; pka = 4.76

SEE MORE QUESTIONS

Recommended textbooks for you

Introductory Chemistry For Today

Chemistry

ISBN:

9781285644561

Author:

Seager

Publisher:

Cengage

Appl Of Ms Excel In Analytical Chemistry

Chemistry

ISBN:

9781285686691

Author:

Crouch

Publisher:

Cengage

Chemistry & Chemical Reactivity

Chemistry

ISBN:

9781337399074

Author:

John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Publisher:

Cengage Learning

Introductory Chemistry For Today

Chemistry

ISBN:

9781285644561

Author:

Seager

Publisher:

Cengage

Appl Of Ms Excel In Analytical Chemistry

Chemistry

ISBN:

9781285686691

Author:

Crouch

Publisher:

Cengage

Chemistry & Chemical Reactivity

Chemistry

ISBN:

9781337399074

Author:

John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Publisher:

Cengage Learning

SEE MORE TEXTBOOKS

If you want to set up an enzyme reaction. You have the following, 10X buffer 10U/ul enzyme 50 ng/ul DNA Water. You want a final 40 ul reaction with 250 ng of DNA, 1x buffer, and 2 units of Enzyme. How much of each of the above do you need?