If we wanted to measure the BSA absorbance without the Biuret reagent, which wavelength would we use and why?
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If we wanted to measure the BSA absorbance without the Biuret reagent, which wavelength would we use and why?
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- Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement or during the transfer to the spectrophotometer?The BSA stock solution from the previous problem was then diluted to generate a set of standard solutions of known concentrations. After performing biuret assay on these solutions, their absorbance at 540 nm were measured using a UV-vis spectrophotometer. The following data were obtained. Concetration of BSA Absorbance (mg/mL) 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8 0.380 1.0 0.485 Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.
- The two most abundant pigments in the chromatography results were carotene and Xanthophyll. What colors are absorbed or reflected by various pigments and how much is there?Dilute silver nitrate is sometimes applied to the eyes of newborn infants. Explain why a dilute solution is used rather than concentrated.What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?
- In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.The data from thin layer chromatographic separation of analgesics showed ibuprofen distance to be 0.752 mm. If the solvent distance is 1.85 mm. Calculate the retention factor for Ibuprofen?Given the following absorbance spectrum, to what wavelength should you set the spectrophotometer to measure your samples? Absorbance 400 500 600 Wavelength (nm) 700
- When conducting a flame test for sodium iodide, is the result positive or negative?Sulfamethoxazole is a type of sulfonamide antibiotic. Its absorbance ranges from 250-300 nm in a UV spectrophotometer, which tells that it is in the UV region. What characteristic/s of sulfamethoxazole is responsible for the absorbance in the UV region? Please answer in detail and clearly. Thank you!The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?
