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Q: Multiplexing agglutination
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- https://youtu.be/w7aIxiZQ60g Multiplexing agglutination https://youtu.be/uWStmyJ5Qc0 This is the multiplexing agglutination. Lab report I don’t really know what to talk about, the data, conclusions and the purpose of this. Need help pleaseWhich portion of the blood is used for the Elisa test? Explain.Counterstaining with Hematoxylin and Eosin is an important step in which of the following techniques? ELISA Gel Electrophoresis IHC Western blotting
- Why does the antibody titer determination use twofold dilutions ofthe antiserum rather than 10-fold dilutions?Define Blue/White Screening. Explain principle mechanism of this approach briefly.The following pictures show the results of a Disk Diffusion Assay for different types of bacteria. For each bacteria, what antibiotic would you recommend be used on the patient? Explain your choice.
- What are the ordered steps of an ELISA protocol? A. Add primary antibody->wash-> Bind sample to a surface ->Add substrate ->Add secondary antibody-enzyme conjugate ->wash B. Bind sample to support -> Add substrate -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash C. Bind sample to a surface -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash -> Add substrate D. Add secondary antibody-enzyme conjugate -> wash -> Add primary antibody -> wash -> Add substrate -> Bind sample to surfaceGive five (5) other examples of samples that are best prepared using Smear Preparation Technique (example: Human RBCs). Explain why.You are tasked with measuring the quantity of prostate specific antigen (PSA) in a urine sample using an ELISA strategy. Describe and schematically depict how you would perform the test given the following materials: anti-PSA, biotinylated anti-PSA, streptavidin-HRP, HRP substrate kit (HRP = horse radish peroxidase).
- true or false Primary cells are isolated directly from tissues and have not been modified ex vivo using an immortalization protocol.What does a white colony indicate during blue-white screening? Explain how the color is formed.QUESTION 2 How does Complement fixation test differ from haemolytic titration assay for complement? (Answer should not be more than three lines). QUESTION 3 ELISA technique was used to assay all three pathways of complement. Which molecule will be adsorbed to the ELISA plate in each of the pathways. QUESTION 4 How can radial immunodiffusion be used to demonstrate complement function assay? (Answer should not be more than two lines) QUESTION 5 A 3-year-old child has a history of serious infections and is currently hospitalized with meningitis. The doctor suspects that he may have a complement deficiency and orders testing. A buffer that chelates calcium was added to specimen. There was a decrease radial haemolysis on agarose plate a) Which complement pathway accounted for the result obtained and how will the result in terms of hemolysis at 50 %? b) What is hydrodynamic focusing and why is it an important feature of an instrument.