If ferrocene was contaminated with some acetylferrocene after the column, what could you do to purify it further? 4. Could you use a column to separate a mixture of 3 different compounds? Why or why not? 5. Reverse phase chromatography is a type of column where the stationary phase is very nonpolar. If you were to run a reverse phase column on ferrocene and acetylferrocene, in what order would they elute? How could you test the purity of your samples after the column?
1. Report your observations – the picture of your final TLC plate, the calculated Rf values
and the identity of each spot.
Rf values ferrocene: 0.875
Rf values acetylferrocene: 0.625
2. Was your ferrocene and acetylferrocene pure after the column? How do you know?
3. If ferrocene was contaminated with some acetylferrocene after the column, what could
you do to purify it further?
4. Could you use a column to separate a mixture of 3 different compounds? Why or why
not?
5. Reverse phase chromatography is a type of column where the stationary phase is very
nonpolar. If you were to run a reverse phase column on ferrocene and acetylferrocene,
in what order would they elute? How could you test the purity of your samples after the
column?
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