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Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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Solved in 2 steps
- 1. Using the DNA provided transcribe DNA into MRNA. 2. Use the mRNA strand you created and break it up into codons. 3. Plug the codons into the amino acid chart to determine the correct amino acid needed to build that protein. 4. Identify the protein you made by comparing the sequence to the pictures 5. Answer the questions for each protein molecule you build before moving on to the next. Protein 1: DNA AAGACCGTATAC MRNA Amino Acid Sequence 1. Which kind of protein molecule did this gene make? 2. How does this protein help the body maintain homeostasis?Scientists can put spider genes into yeast. The yeast can decode the spider genes to make spider silk protein. Why is this so? Evaluate the models. MODEL 1 Spider DNA mRNA mRNA Spider Protein 3. The yeast used machinery from a spider to read the spider genetic code, so both organisms make a protein that is identical. 1. The yeast transcription machine cannot read the spider gene, so yeast uses a machine from a spider to make an mRNA copy. Transcription Machine 2. The yeast translation machine cannot read the mRNA, so yeast uses a machine from a spider to make the protein. ◄ Translation Machine MODEL 2 Spider DNA ▼ mRNA mRNA Spider Protein 3. Yeast and spiders use the same genetic code, so both organisms make a protein that is identical. 2. Explain why one model is correct and the other one isn't. 1. Which model correctly explains what happens inside the yeast cell? 1. The yeast transcription machine reads the spider gene and makes an mRNA copy. Transcription Machine 2. The yeast…Directions: 1. Use the DNA code to make a replicated copy of DNA. 2. Use the replicated DNA code to create your transcription mRNA code. 3. Use the mRNA code to create your tRNA anticodon. 4. USE THE MRNA CODE TO DETERMINE YOUR AMINO ACIDS. MAKE SURE TO PUT YOUR LETTERS IN ALL CAPS! DNA ATG GCA AGC GCT TGA DNA TAC REPLICATION DNA TO MRNA AUG TRANSCRIPTION MRNA to tRNA UAC ANTICODON MRNA to AMINO AUG (START) (STOP) ACID TRANSLATION DNA ATG ССА GAC ACG TGA DNA ТАС REPLICATION DNA TO MRNA AUG TRANSCRIPTION MRNA to TRNA UAC ANTICODON MRNA to AMINO AUG (START) (STOP) ACID TRANSLATION
- Part II. Give what is needed. |Original DNA Sequence: TACACCT T G G C G A C G A C T MRNA Sequence: | Amino Acid Sequence: Mutated DNA Sequence #1: T A C A T C T T G G C GAC GA C T What's the mRNA sequence? (Circle the change) What will be the amino acid sequence? . Will there likely be effects?. What kind of mutation is this? Mutated DNA Sequence #2: T A C G AC C T T G G C G A C G AC T What's the mRNA sequence? (Circle the change) What will be the amino acid sequence?. Will there likely be effects?. What kind of mutation is this? Mutated DNA Sequence #3: T A C A C C T T A G C GAC GA C T What's the mRNA sequence? (Circle the change). What will be the amino acid sequence?. Will there likely be effects? | What kind of mutation is this? Mutated DNA Sequence #4: T A C A C C T T G G C GACTAC T What's the mRNA sequence? (Circle the change), What will be the amino acid sequence?. Will there likely be effects? - What kind of mutation is this? Mutated DNA Sequence #5: T A C A C C T T G G G A C…The last two questions 4 and 5 The last two questions 4 and 5 The last two questions 4 and 5 Use the mRNA coding chart above to answer these questions. he following is the base sequence on a portion of a template strand of DNA. 3 ‘ TACGCCAGTGGTTCGCAC 5’ Give the base sequence of the complementary DNA strand. Give the base sequence of the strand of mRNA read from the original DNA template strand. List, in order, the amino acids that would be present in this protein? 4. What would be the code that the methionine tRNA anticodon would carry? 5. Suppose a mutation altered the original DNA strand so that the 6th nucleotide was changed to a T i) How would this change the protein? ii) What type of mutation is this?Now imagine that a mutation occurred at the first G in the DNA sequence above and the G became a C. How would this affect the mRNA and the amino acid for that codon? Old DNA codon Old RNA codon Old amino acid New DNA codon New mRNA codon New amino acid T T G T T C This would be an example of which type of a point mutation?__________________________
- Which choice best fits the blank? Refer to picture. The ribosome moves along the mRNA strand. In panels b, c, and d, new tRNAs carrying ___________. match up with the codons of the mRNA strand. After each tRNA locks into place, a peptide bond forms between the amino acid the tRNA is carrying and the amino acid already there. This process repeats until the end of the sequence is reached. A. ProteinsB. NucleotidesC. Amino AcidsThe following sequence is the coding strand of a piece of DNA. Type out the corresponding template strand of DNA and the MRNA that could be made from this piece of DNA if you assume that transcription begins at the first nucleotide listed. Next, use the attached genetic code table to translate the mRNA into a polypeptide that could be made from the message and list that sequence along with those of the DNA and MRNA. Coding strand 5'-TACCGTATGATTCTCTTGTATGGGTAACC-3' Second letter U UUU UUC UCU UAU UGU Phe Tyr Cys UCC UCA UAC UAA STOP UAG STOP UGG| Trp UGC Ser UUA UGA STOP A UUG Leu UCG CUU CCU CC CAU CGU CGC His CUC C CUA CAC САА Leu Pro Arg CCA CGA Gln CUG СCG CAG CGG AGU Ser AAU AAC ACU AUU lle AUC Asn ACC ACA AGC Thr AAA AGA AUA AUG Met Arg ACG AAG Lys AGG GAU GCU GCC GUU GGU Asp GAC GGC GGA GUC Val Ala Gly GCA GCG GAA GAG GUA Glu GUG GGG Third letter UCAG UCAG 5CAG UCAG First letterBelow is the coding strand of DNA. Transcribe it to the proper mRNA: 5' ATTCGCTAGATCA3 How do I do a diagram on this
- The following is a prokaryotic DNA sequence. Fill in the other strand of DNA. Be sure to label the ends appropriately (5’ and 3’). Transcription goes from left to right and stops after the right most base pair. On your DNA, indicate which is the coding/partner and which one is the template. Write out the pre-mRNA transcript. Be sure to label the ends appropriately (5’ and 3’). DNA 5’ – T G G G G G A T A C C G C A T G C A G G T A G T C T A A G C G A G T G A C – 3’ DNA mRNA1. Given the piece of MRNA: 5'-CCUGCAGUAUGAAACGCCUGGUAGAAGGUGGGAAGUGGUGCGCC-3' 1. Predict the DNA strand sequence from which it was transcribe 2. List the complementary non-coding DNA Sequence 3. List the Amino acid Sequence of the protein coded for. Use the Genetic Code 2. Below is a short segment of a DNA molecule. Translate the DNA codon into MRNA. 5'- TACCATGAGAATTGTGG TCACCTTTTT-3' 3'- ATGGTACTCTTAACACCAGTGGAAAA A-5' MRNA Sequence Amino Acid SequenceNow imagine that a mutation occurred in the g of the codon below and the G became a C. How would this affect the mRNA and the amino acid for that codon? Old DNA codon Old RNA codon Old amino acid New DNA codon New mRNA codon New amino acid A T G A T C This would be an example of which type of a point mutation?__________________________