If a cell were given glucose labeled at carbon-3 with 14C, what carbon(s) of pyruvate would contain the label? 1 2 3 1 and 3 O2 and 3
If a cell were given glucose labeled at carbon-3 with 14C, what carbon(s) of pyruvate would contain the label? 1 2 3 1 and 3 O2 and 3
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:**Question:**
If a cell were given glucose labeled at carbon-3 with ¹⁴C, what carbon(s) of pyruvate would contain the label?
**Options:**
- ○ 1
- ○ 2
- ○ 3
- ○ 1 and 3
- ○ 2 and 3
**Explanation of the Question:**
This question tests your understanding of the glycolytic pathway, specifically how glucose is metabolized into pyruvate. Labeling carbon-3 of glucose with ¹⁴C allows for tracing of this carbon atom through the metabolic pathway.
**Analysis:**
- During glycolysis, glucose is cleaved into two molecules of glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP), which are interconverted. The process ultimately leads to two molecules of pyruvate.
- Each original carbon from glucose is distributed in specific patterns in the resulting pyruvate molecules.
- Understanding the steps will help identify which carbon(s) in pyruvate corresponds to the labeled carbon in glucose.
**Applications in Education:**
- This example can demonstrate the importance of carbon tracing in metabolic pathways.
- It highlights the complexity of cellular respiration and the fate of atoms during biochemical reactions.
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