If a cell were given glucose labeled at carbon-3 with 14C, what carbon(s) of pyruvate would contain the label? 1 2 3 1 and 3 O2 and 3

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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**Question:**

If a cell were given glucose labeled at carbon-3 with ¹⁴C, what carbon(s) of pyruvate would contain the label?

**Options:**

- ○ 1
- ○ 2
- ○ 3
- ○ 1 and 3
- ○ 2 and 3

**Explanation of the Question:**

This question tests your understanding of the glycolytic pathway, specifically how glucose is metabolized into pyruvate. Labeling carbon-3 of glucose with ¹⁴C allows for tracing of this carbon atom through the metabolic pathway.

**Analysis:**

- During glycolysis, glucose is cleaved into two molecules of glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP), which are interconverted. The process ultimately leads to two molecules of pyruvate.
- Each original carbon from glucose is distributed in specific patterns in the resulting pyruvate molecules.
- Understanding the steps will help identify which carbon(s) in pyruvate corresponds to the labeled carbon in glucose.

**Applications in Education:**

- This example can demonstrate the importance of carbon tracing in metabolic pathways.
- It highlights the complexity of cellular respiration and the fate of atoms during biochemical reactions.
Transcribed Image Text:**Question:** If a cell were given glucose labeled at carbon-3 with ¹⁴C, what carbon(s) of pyruvate would contain the label? **Options:** - ○ 1 - ○ 2 - ○ 3 - ○ 1 and 3 - ○ 2 and 3 **Explanation of the Question:** This question tests your understanding of the glycolytic pathway, specifically how glucose is metabolized into pyruvate. Labeling carbon-3 of glucose with ¹⁴C allows for tracing of this carbon atom through the metabolic pathway. **Analysis:** - During glycolysis, glucose is cleaved into two molecules of glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP), which are interconverted. The process ultimately leads to two molecules of pyruvate. - Each original carbon from glucose is distributed in specific patterns in the resulting pyruvate molecules. - Understanding the steps will help identify which carbon(s) in pyruvate corresponds to the labeled carbon in glucose. **Applications in Education:** - This example can demonstrate the importance of carbon tracing in metabolic pathways. - It highlights the complexity of cellular respiration and the fate of atoms during biochemical reactions.
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