HPO, 10- | HPO, pH pK +1 8- pK +1- (H¿PO]] = [HPO,"] pK = 7.2 Buffer pH 7- 7.2 = pK,- pK, -1- region 6- PK – 1 Inflection point H„PO, H,PO, 4 50 100 % H,PO, in solution % HPO in solution 100 90 50 10 Relative abundance (%) O 10 50 90 100 Н.РО] in excess НРО in excess The titration curve of H,PO, showing the buffer region for the H2PO7/HPO pair. Relative abundance of H,PO, and HPO". FIGURE 2.17 The relationship between the titration curve and buffering action in H,PO,.

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Chapter1: Chemical Foundations
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Look at Figure 2.17. If you did this titration using TRIS instead of phosphate, how would the titration curve look compared to the figure? Explain.

HPO,
10-
| HPO,
pH
pK +1
8-
pK +1-
(H¿PO]] = [HPO,"]
pK = 7.2
Buffer
pH
7-
7.2 = pK,-
pK, -1-
region
6-
PK – 1
Inflection point
H„PO,
H,PO,
4
50
100
% H,PO, in solution
% HPO in solution
100 90
50
10
Relative abundance (%)
O 10
50
90 100
Н.РО] in excess
НРО in excess
The titration curve of H,PO, showing the
buffer region for the H2PO7/HPO pair.
Relative abundance of H,PO, and
HPO".
FIGURE 2.17 The relationship between the titration curve and buffering action in H,PO,.
Transcribed Image Text:HPO, 10- | HPO, pH pK +1 8- pK +1- (H¿PO]] = [HPO,"] pK = 7.2 Buffer pH 7- 7.2 = pK,- pK, -1- region 6- PK – 1 Inflection point H„PO, H,PO, 4 50 100 % H,PO, in solution % HPO in solution 100 90 50 10 Relative abundance (%) O 10 50 90 100 Н.РО] in excess НРО in excess The titration curve of H,PO, showing the buffer region for the H2PO7/HPO pair. Relative abundance of H,PO, and HPO". FIGURE 2.17 The relationship between the titration curve and buffering action in H,PO,.
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