You are given a buffer that is labeled 25x and your instructor asks you to dilute it to 1x in the final tube. If you have a final volume of 500uL in your tube how much of the 25x buffer will you need to add to bring it to 1x? Do you need to add anything else to the tube before using the new solution?

Answered: You are given a buffer that is labeled…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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You are given a buffer that is labeled 25x and your instructor asks you to dilute it to 1x in the final tube. If you have a final volume of 500uL in your tube how much of the 25x buffer will you need to add to bring it to 1x? Do you need to add anything else to the tube before using the new solution?

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The concept used in this question is dilution. Dilution is the process of reducing the concentration of a solution by adding more solvent to it.

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