how to calculate the experiment fow rate for this gel chromatography Experiment flow rate ? Flow rate 4.75mL/min

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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how to calculate the experiment fow rate for this gel chromatography

Experiment flow rate

?

Flow rate

4.75mL/min

Time (sec)
Volume of elution (mL) ( 1%
bromophenol blue)
Corrected OD
1
0.237
2
0.408
3
10
0.342
4
10
0.234
10
0.369
6
13
0.426
7
17
0.231
8
18
0.339
9
14
0.318
10
14
0.231
11
12
0.342
12
15
0.228
13
17
0.333
14
18
0.405
15
15
0.231
Total
201
Transcribed Image Text:Time (sec) Volume of elution (mL) ( 1% bromophenol blue) Corrected OD 1 0.237 2 0.408 3 10 0.342 4 10 0.234 10 0.369 6 13 0.426 7 17 0.231 8 18 0.339 9 14 0.318 10 14 0.231 11 12 0.342 12 15 0.228 13 17 0.333 14 18 0.405 15 15 0.231 Total 201
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