Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
how Recombinant Plasmid Libraries Simplify
Gene Identification?
Introduction
Plasmid Vectors: these are like the artificial vehicle to deliver our gene of insert into our target host and thus we can amplify our gene of insert in Bacteria. Any bacterial cell contains two set of genetic material one is the main genetic material while other one is called as Plasmid DNA. Plasmid DNA is circular double stranded DNA which increase the survivability of the bacteria as it contains the extra copies of various antibiotic resistant genes.
If we introduce our GOI (gene of insert) in the plasmid or vector and if the gene is integrated in host genome then these cells are called Transformant cells, while if there is integration of gene in any other location than MCS (Multiple cloning sites) then these cells are termed as non-transformant. On the other hand, if our GOI is not at all integrate with host cell then these cells are called as untransformed cells.
Step by step
Solved in 2 steps
