how does polyacrylamide gel electrophoresis with SDS separate proteins ?

Q: What is differential centrifugation? Explain how it can be used to separate cellular organelles.

A: Differential centrifugation : A separation method where the components of a cell are separated on…

Q: Why do we need to amplify the DNA at all in order to visualize it in a gel?

A: PCR (polymerase chain reaction ) is a widely used technique to amplify DNA while Gel electrophoresis…

Q: Since higher concentration colcemid will result in shorter chromosome, you want to      change your…

A: It is given that: Molar concentration of the stock solution: 10 ug/ml.  Desired dilution…

Q: Name the technique used to separate fragments of DNA or mixtures of proteins?

A: The gel electrophoresis is a technique to separate DNA fragments and proteins based on their size,…

Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?

A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…

Q: What are nanopores used for and tell How does DNA bind to the nanopore?

A: Deoxyribonucleic acid (DNA) sequencing is the process of determining the nucleic acid sequence in…

Q: What is the source of agarose, the powder used to make the gel?                        What is the…

A: Gel electrophoresis is a technique used by scientists to separate DNA or protein molecules.  Gel…

Q: How does gel electrophoresis separate DNA fragments of your unknown sample and How will you be able…

A:

Q: In the alkaline plasmid screen, what two things separate plasmid from chromosomal DNAs?

A: Following is the procedure for the separation of plasmid by the alkaline lysis method.

Q: What are dyes that stain DNA used for? Is background staining a problem when using such dyes?

A: The fragments of deoxyribonucleic acid (DNA) can be visualized by the dye after the separation…

Q: How do we separate plasmid and chromosomal DNA during the alkaline plasmid screen?

A: Alkaline lysis is the most common method used for separating plasmid DNA from chromosomal DNA.

Q: How are dideoxynucleotide triphosphates utilized in sequencing?

A: The chain elongating inhibitors of the DNA polymerase which are required for the Sanger method of…

Q: Which of the following was added RNA extraction? Buffer RPE Wash buffer guanidine-isothiocyanate…

A: DNA molecule is a sritical methd. It helos in serine,  nRNa Nither momt tertibre

Q: What is in situ hybridization used for and how  does In Situ Hybridization detect?

A: Hybridization, in general, is a process during which a moiety is formed having combined traits of…

Molecular Techniques

Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.

DNA Fingerprinting and Gel Electrophoresis

The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.

Molecular Markers

A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker. 

DNA Sequencing

The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.