How do you determine concentration of an unknown using standard addition method? For example, if you have five solutions with varying amounts of the unknown, 0 mL, 10 mL, 20 mL, 30 mL, and 40 mL, per 100 mL of 0.05 M solution and absorbance values for each, how would you use this to find concentration of the unknown. Would the unknown concentration be the slope of the standard addition model graph, or do you have to determine concentration of all five solutions and use these concentrations and the absorbance values to create an absrobance vs concentration graph? If so, would the slope of that graph be the concentration of the unknown.

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