How can I explain the results of my findings in lamen terms?
1 - Name of Test Type of Medium |
2 - Color Before Incubation |
3-Color After Incubation? |
4 – Results Positive/ Negative? |
5 - Reagents Added |
Lactose Fermentation test Phenol Red Lactose (PRL) |
Red |
Color: Yellow
Gas Bubble: Yes |
Positive |
Phenol Red to detect color change (added) |
Glucose Fermentation Test Phenol Red Glucose (PRG) |
Red |
Color: Yellow
Gas Bubble: Yes |
Positive |
Phenol Red to detect color change (added) |
Citrate Utilization Simmons Citrate |
Green |
Color: Blue |
Positive |
Bromthymol Blue (added) |
Indole Test Media = SIM |
Clear |
Indole: Color: Clear |
Negative |
For Indole, we add Kovac’s
|
Hydrogen Sulfide Test Media = SIM |
clear |
Hydrogen Sulfide: Color: Clear |
Negative |
Iron already added |
Methyl Red Test (MR test) Media = MRVP Broth |
Clear |
Color: Yellow |
Negative |
We add Methyl Red Reagent |
Voges-Proskauer Test (VP test) Media = MRVP Broth |
Clear |
Color: Pink coloration |
Positive result: Alcohols are produced. |
We add Barritt’s reagent |
Eosin Methylene Blue Test Media = EMB agar
|
Reddish – no growth |
Color: Purple to black colonies |
These test are the perfomed to identify positive and negative result for glucose fermentation.
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