hosphate buffered saline (PBS) is a buffer solution commonly used in biological The buffer helps to maintain a constant pH. The osmolality and ion concentrations of the solution usually match those of the human body.You need to prepare a stock solution at pH 7.00 with NaH2PO4 and Na2HPO4 (pKa =7.21). What would be the respective concentration of these substances if you wish to obtain the final phosphate concentration [HPO4 −2] + [H2PO4 −] = 0.3 M? What will be the pH of the solution after adding 0.18 L of 0.2 M NaOH? What is the ratio of HPO4−2 of H2PO4 – is part b, and what is the significance? NON GRADED PRACTICE QUESTION FOR BIOCHEM

The ionization process of concern here is represented below.H2PO4- HPO4-2 + H+Here, H2PO4- is the…

Answered: hosphate buffered saline (PBS) is a…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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hosphate buffered saline (PBS) is a buffer solution commonly used in biological The buffer helps to maintain a constant pH. The osmolality and ion concentrations of the solution usually match those of the human body.

You need to prepare a stock solution at pH 7.00 with NaH₂PO₄ and Na₂HPO₄ (pKa =7.21). What would be the respective concentration of these substances if you wish to obtain the final phosphate concentration [HPO₄ ⁻²] + [H₂PO₄ ⁻] = 0.3 M?
What will be the pH of the solution after adding 0.18 L of 0.2 M NaOH?
What is the ratio of HPO₄⁻² of H₂PO4 ^– is part b, and what is the significance?

NON GRADED PRACTICE QUESTION FOR BIOCHEM

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