Hopkin Cole's Test Observations Albumin Gelatin Casein
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Hopkin's Cole Test (Indole ring) Results

Transcribed Image Text:C. Hopkin's Cole Test (Indole ring)
1. Place 1 mL of each gelatin, albumin, and casein solution in separately labeled test
tubes.
2. Add four drops of Hopkin's Cole reagent.
3. Incline the test tube in the test tube rack.
4. Carefully, allow 2 mL of conc. sulfuric acid to slide down the side of the inclined
tube so that it will form a layer below the protein solution.
5. Take note of the color junction of the two liquids.
Hopkin Cole's Test
Observations
Albumin
Gelatin
Casein
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