Hopkin Cole's Test Observations Albumin Gelatin Casein
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A: help to detect the soluble protein.
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- Give the color reactions. Test Tube 1. starch solution, pancreatin, and water 2. star solution, pancreatin, and sodium carbonate Lugol's Test Benedict's Test 3. starch solution Note: Test tubes are immersed in a water bath for 30 minutes in temperature 37 degrees celsius to 40 degrees celsius before performing the 2 tests. Question: What test tube will show positive starch hydrolysis and negative starch hydrolysis?What is the purpose of Xanthoproteic test and explain the observation or rection for albumin and gelatin in terms of principle of xanthoproteic test.What is the color result of 1% soultion of casein,albumin ang gelatin in biuret test? Results and interpretation with reference
- Methyl red test Does a Phenol red organism have to be methyl red?What is the color result of 1% solution of casein, Albumin, Gelatin in ninhydrin test? Include picture and interpretation With referenceGive the color reactions. Test Tube 1. starch solution, pancreatin, and water 2. starch solution, pancreatin, and sodium carbonate Lugol's Test Benedict's Test 3. starch solution Note: Test tubes are immersed in a water bath for 30 minutes in temperature 37 degrees celsius to 40 degrees celsius before performing the 2 tests. Questions: 1. What test tube will show positive starch hydrolysis and negative starch hydrolysis? Explain each why it resulted in positive starch hydrolysis and negative starch hydrolysis. 2. What will be the effect of sodium carbonate in the test tubes?
- MOLLISCH TEST You can use this as your reference : https://youtu.be/rKng5-ij6kQGELATIN b.how will you inoculate? c.How will you incubate? d. what are Post- Incubation procedures? e. How will you read the positive and negative results?2. Mucic acid test – place 1 ml of the sample (lactose, galactose, glucose, fructose) in separate tubes. Add 1 ml conc. HNO3 in each tube. Heat the tubes in a boiling water bath for 1 hour. Cool at room temperature. Induce crystallization by scratching the side of the tube with a clean stirring rod, if no crystals appear; let it stand until the next laboratory period. Examine the crystals under the microscope and draw.
- Answer TRUE OR FALSE only. 1. All types or kinds of media powder has a standard ratio of 23:1000. Meaning that 1000 ml of water is needed to dissolve 23 grams of powder medium. 2. Simple stains use one dye and cannot differentiate various types of bacteria while differential stains use several dyes in order to differentiate between different kinds of bacteria. 3. A certain student prepared his fixed sample for examination by pouring Nigrosin which is an acidic stain. He then observed that under the microscope the background was stained but the bacterial cells were untouched. This student therefore adapted the acid-fast staining procedure. 4. In doing serial dilutions, the original sample must be shaken at least 25 times to obtain a uniform distribution of organisms. 5. Label all reagents with its name, concentration and date of its preparation except for water.PRECIPITATION OF PROTEINS How will the different agents precipitate egg albumin? Include chemical equations involved Heat Heavy metal salts Strong mineral acids Full-saturation test half-saturation test Alkaloidal reagents alcoholHow is protein-free alkaloidal extract obtained for subsequent testing? 2.What is a pseudoalkaloid?