he following text may be found at the ThermoFisher website: "PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. Each 10X PBS solution is ready to use upon dilution to the desired concentration." This means that the product that you purchase has a concentration 10 times the concentration at which it is used. 1. Look up the pKa values for phosphoric acid and list them as a part of your response. 2. Based on the description of the contents of the PBS buffer, calculate the pH of the 1X solution. 3. Assume that you wish to use a pH = 7.00 buffer. If your answer to 2 was more acidic than pH 7.00, calculate how much 1.00 M NaOH you would need to add to get to pH = 7.00. If your answer to 2 was more basic than pH 7.00, calculate how much 1.00 M HCl you would need to add to get to pH = 7.00.
The following text may be found at the ThermoFisher website: "PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. Each 10X PBS solution is ready to use upon dilution to the desired concentration." This means that the product that you purchase has a concentration 10 times the concentration at which it is used.
1. Look up the pKa values for phosphoric acid and list them as a part of your response.
2. Based on the description of the contents of the PBS buffer, calculate the pH of the 1X solution.
3. Assume that you wish to use a pH = 7.00 buffer. If your answer to 2 was more acidic than pH 7.00, calculate how much 1.00 M NaOH you would need to add to get to pH = 7.00. If your answer to 2 was more basic than pH 7.00, calculate how much 1.00 M HCl you would need to add to get to pH = 7.00.
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