sure to answer all parts. nicrobiologist is preparing a medium on which to culture E. coli bacteria. She buffers the med € 7.00 to minimize the effect of acid-producing fermentation. What volumes of equimolar aque utions of K₂HPO4 and KH₂PO4 must she combine to make 450.0 mL of the pH 7.00 buffer? values for phosphoric acid: = 7.2 × 10-³

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Chapter1: Chemical Foundations
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A microbiologist is preparing a medium on which to culture E. coli bacteria. She buffers the medium at
pH 7.00 to minimize the effect of acid-producing fermentation. What volumes of equimolar aqueous
solutions of K₂HPO4 and KH₂PO4 must she combine to make 450.0 mL of the pH 7.00 buffer?
Ka values for phosphoric acid:
Kal = 7.2 × 10-3
Ka2= 6.3 × 10-8
Ka3 = 4.2 × 10-13
Volume H₂PO4 =
Volume HPO42
=
mL
mL
Transcribed Image Text:Be sure to answer all parts. A microbiologist is preparing a medium on which to culture E. coli bacteria. She buffers the medium at pH 7.00 to minimize the effect of acid-producing fermentation. What volumes of equimolar aqueous solutions of K₂HPO4 and KH₂PO4 must she combine to make 450.0 mL of the pH 7.00 buffer? Ka values for phosphoric acid: Kal = 7.2 × 10-3 Ka2= 6.3 × 10-8 Ka3 = 4.2 × 10-13 Volume H₂PO4 = Volume HPO42 = mL mL
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