Tris(hydroxymethyl)aminomethane, or TRIS as it is commonly known, is a buffer oftenused in biochemical work. Kb = 1.19 x 10-6for(HOCH2)3CNH2 + H2O <-> (HOCH2)3CNH3+ + OHA buffer is prepared by combining 50.0 g TRIS base and 65.0 g TRIS HCl in 2.0 L water.What is the pH of this buffer? What is the pH after 0.50 mL of 12 M HCl is added to 200.0 mLof buffer solution?

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Tris(hydroxymethyl)aminomethane, or TRIS as it is commonly known, is a buffer often
used in biochemical work. Kb = 1.19 x 10-6
for
(HOCH2)3CNH2 + H2O <-> (HOCH2)3CNH3
+ + OHA buffer is prepared by combining 50.0 g TRIS base and 65.0 g TRIS HCl in 2.0 L water.
What is the pH of this buffer? What is the pH after 0.50 mL of 12 M HCl is added to 200.0 mL
of buffer solution?

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